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Activation Assay Special Offer

21/03/2018 11:52:28 AM

Since 2001, Cytoskeleton has provided the scientific communitywith the most robust, accurate, and time-saving kits to measureSmall GTP-binding protein (SmG) activation. Along the way, wehave developed numerous versions for different SmGs, such as Rho, Rac, Arf1 & 6, Ras, and Ral. Also, the quantifiable GLISAversions enabled a new wave of more sensitive applications, e.g.measurement in limited primary cell numbers and Matrigel 3Dmatrix. We continue to develop and maintain these high standards,which allow you to produce the best results in the least amount oftime.SmGs are involved in regulating cell signaling pathways and impacta wide range of cellular processes, functions, and morphology.The Pulldown version of the assay uses affinity beads which areincubated with the extract and then separated by centrifugation.The pelleted products are separated by SDS-PAGE and blottedonto a membrane for western analysis of the SmG of interest. TheGLISA® format is a modified ELISA which has the affinity reagentpermanently attached to the well of a 96-well plate. The extract is incubated in the well which is then washed and probed withprimary and secondary antibodies.

User-Friendly Formats

Accurate and Sensitive

Over 500 Citations

 

Applications for G-LISAs 

Swiss 3T3 fibroblasts were treated with 1 μg/ml CN04 for the indicated times and cell lysates were subjected to G-LISA activation assays for RhoA (Cat.# BK124), Rac1 (Cat.# BK128), and Cdc42 (Cat.# BK127).

Swiss 3T3 fibroblasts were serum starved for 24h, then either treated with 10 ng/ml of EGF for 2 min (Lanes 4 & 5) or left untreated (Lanes 2 & 3).  Rac1 activation was measured using the Rac1 Activation pull-down assay (Cat. # BK035) (Lanes 2-5).  Lane 1 is 20 ng of recombinant Rac1-His protein.

 

Measure active GTPases in 3-D cell cultures

  • Pull-down assays require a prohibitive amount of cell lysate

Measure active GTPases in primary cell lysates

  • Pull-down assays require >20-fold more lysate

Increase accuracy of active GTPase quantitations

  • Pull-down assays are analyzed by semi-quantitative western blotting

Measure active GTPases in tissue lysates

  • Pull-down assays require >20-fold more lysate

Applications for Pull-downs

Measure active GTPase isoforms 

  • Active levels of multiple Ras- and Rho-subfamily GTPase isoforms can be detected

Complement G-LISA findings 

  • Use two different, validated techniques to quantitate active GTPase levels

Cited in hundreds of peer- reviewed articles.

3-D Cell Culture and G-LISAs: 

 

Keely P.J. et al. 2007. Investigating integrin regulation and signaling events in three-dimensional systems. Methods Enzymol. 426, 27-45. 

 

Ponik S.M. et al. 2013. RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis. Mol. Biol. Cell. 24, 1688-1699. 

 

Petroll W.M. et al. 2008. Dynamic assessment of fibroblast mechanical activity during Rac-induced cell spreading in 3-D culture. J. Cell Physiol. 217, 162-171. 

 

Primary Cells and G-LISAs: 

 

Valdez C.M. et al. 2016. The Rac-GAP alpha2-chimaerin regulates hippocampal dendrite and spine morphogenesis.  Mol. Cell. Neurosci. 75, 14-26. 


Nini L. and Dagino L. 2010. Accurate and reproducible measurements of RhoA activation in small samples of primary cells. Anal. Biochem. 398, 135-137. 


Flaiz C. et al. 2009. PAK kinase regulates Rac GTPase and is a potential target in human schwannomas. Exp. Neurol. 218, 137-144. 

 

Increased accuracy and G-LISAs:

 

Oliver A.W. et al. 2011. The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42. Br. J. Cancer. 104, 324 - 331. 

 

GTPase isoforms and Pull-downs:

 

Tabibian J.H. et al. 2014. Cholangiocyte senescence by way of N-Ras activation is a characteristic of primary sclerosing cholangitis. Hepatology. 59, 2263-2275. 


Kusuhara S. et al. 2012. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothe­lial cells. PLoS ONE. 9:e45858. 

 

Pull-downs complement G-LISAs:

 

Tanaka T. et al. 2010. Monocyte chemoattractant protein-1/CC chemokine ligand 2 enhances apoptotic cell removal by macrophages through Rac1 activation. Biochem. Biophys. Res. Commun. 399, 677-682. 

 

Schlegel N. and Waschke J. 2009. VASP is involved in cAMP-mediated Rac 1 activation in microvascular endothelial cells. Am. J. Physiol. Cell Physiol. 296, C453-C462. 

 

Lai F.P.L. et al. 2009. Cortactin Promotes Migration and Platelet-derived Growth Factor-induced Actin Reorganization by Signaling to Rho-GTPases. Mol. Biol. Cell. 20, 3209-3223. 

 

Click here for more examples (see Citation tab).

 

 

 

cytoactiassay

It's with pleasure that we inform you that LDN has launched today its latest products:

  1. an ELISA for the determination of MELATONIN in plasma and serum (catalogue no. BA E-3300)
  2. an ELISA for the direct determination of MELATONIN in saliva (catalogue no. BA E-3400).

 

Following some of the main advantages:

  Elegant and efficient extraction procedure for serum/plasma samples

  Direct measurement of saliva samples

  Highly specific antisera

  Wide standard ranges, convenient measurement of samples without pre-dilution

  Suitable to seize the circadian rhythm including the nocturnal, individual peak

  Quality – produced according to EN ISO 13485 and 9001

form more information and a quote, please contact us at info@huntingtree.co.nz 

 

Detecting endogenous SUMO 2/3 modifications has never been easier

Physiologic Detection: Cytoskeleton's comphrehensive SUMO 2/3 detection kit (Cat. # BK162) simplifies endogenous SUMO 2/3 detection.

sumo

Legend: Untreated, Taxol 100nM, or serum restricted (0% FBS) treated A431, or Hela cells were lysed with BlastR lysis buffer (Cytoskeleton Inc.) supplemented with SUMO isopeptidase (deSUMOylase) inhibitor (NEM). 1mg of each lysate was incubated with the recommended amount of SUMO 2/3 affinity beads (ASM24-beads) or IgG control beads (CIG01) (Cytoskeleton Inc.). Immunoprecipitated samples were separated by SDS-PAGE and transferred to PVDF. Western blot was performed with RhoGDI.

 

Maximize your ability to detect SUMO 2/3 modifications of your target protein

Use the right tools for your SUMO 2/3 investigation

Affinity reagents:  Robust enrichment, capable of endognous SUMO 2/3 detection

Lysis buffers:  Inactivates de-SUMOylases (SUMO isopeptidases) through denaturation

Inhibitors:  Target and inactivate de-SUMOylases

Western antibody:  High sensitivity, detect low % PTMs

Detection Reagents:  Exceptional signal, detect low % PTMs 

Legend: Untreated A431 cells were lysed with BlastR lysis buffer (Cytoskeleton Inc.) supplemented with or without deSUMOylase inhibitor (NEM). 1mg of each lysate was incubated with the recommended amount of SUMO 2/3 affinity beads (ASM24-beads) (Cytoskeleton Inc.). Immunoprecipitated samples were separated by SDS-PAGE and transferred to PVDF. Western blot was performed with SUMO 2/3 antibody (ASM23-HRP), TFII-I, or Ubc9.

For more tips to maximize SUMO 2/3 detection, see our SUMO 2/3 white paper

 

Signal Seeker Acetyl-Lysine Detection Tools

 

(BK162) Signal-Seeker SUMOylation 2/3 Detection Kits (30 Assays)  

 

(BK 162-S)  Signal-Seeker SUMOylation 2/3 Detection Kits (10 Assays)

 

(ASM24-beads) SUMOylation 2/3 Affinity Beads

 

(CIG01-beads) Mouse IgG Control Beads

 

(ASM23) SUMOylation 2/3 Mouse Monoclonal Antibody 

 

(ASM23-HRP) SUMOylation 2/3-HRP labeled Mouse Monoclonal Antibody

 

(BLR01) BlastR Rapid Lysate Prep Kit

SLC - Solute Carrier Superfamily

23/11/2017 11:03:45 AM

SLC - Solute Carrier Superfamily

Solute carriers (SLC) are a group of membrane transport proteins, comprising over 300 members, most of which are located in cell membranes. Their main function is to facilitate the transport of a wide variety of substrates across biological membranes, including the uptake of small molecules into cells.

The SLC superfamily of solute carriers is the second largest family of membrane proteins after G protein-coupled receptors. Within the superfamily, there is a great diversity of structure, but they are united by their function. Over 50 families have been identified based on sequence similarities, but many of them overlap in terms of the solutes that they carry. Two families (SLC3 and SLC7) only generate functional transporters as heteromeric partners, where one partner is a single trans-membrane domain protein. Functionally, members can be divided into those dependent on gradients of ions (particularly sodium, chloride or protons), exchange of solutes or simple equilibrative gating. For many members, the stoichiometry of transport is not yet established. One family of transporters also possess enzymatic activity (SLC27), while many members function as ion channels (e.g. SLC1A7/EAAT5), which increases the complexity of function of the SLC superfamily.

Membrane Transporters

Membrane transporters are widely expressed throughout the body, in the epithelia of major organs, such as the liver, intestine, kidney, and organs with barrier functions, such as the brain, testes and placenta. Different transporters are localised to the plasma membrane, as well as to the membranes of various subcellular organelles. This maintains cellular homeostasis due to the regulated delivery of required substrates. SLC transporters therefore have important roles in physiological processes ranging from the cellular uptake of nutrients to the absorption of drugs and other xenobiotics.

ATP-binding cassette (ABC) Superfamily

Traditionally, a greater emphasis has been placed on the development of drugs targeted at the other transporter superfamily, the ATP-binding cassette (ABC) superfamily. Fewer therapeutic drugs have been developed to exploit SLC family targets but interest in this diverse family of proteins is now growing. Several classes of marketed drugs now target well-known SLC transporters, such as neurotransmitter transporters, and human genetic studies have provided powerful insight into the roles of more-recently characterised SLC transporters in both rare and common diseases, indicating a wealth of new therapeutic opportunities.

We currently offer the following targets, with more in development:

for more information please contact us or check out the website at www.biorbyt.com 

Fibronectin and Laminin

17/11/2017 10:48:41 AM

Fibronectin and Laminin constitute the majority of the dynamic portion of the extracellular matrix of tissues and they are essential components of the culturing technique of many cell types. At Cytoskeleton, we pride ourselves on producing highly pure and biologically active proteins, and in this regard we have optimized fluorescent ECM proteins to contain sufficient dye labeling to be easily observable, while at the same time maintaining full biological activity. There are so many applications of these reagents, some of which are highlighted below.  

 rhodamine fibronectin

MCF10A cells incubated with Rhodamine Fibronectin

Legend: Rhodamine fibronectin (Cat. # FNR01) treated MCF10A cells (image kindly provided by A. Varadaraj and M. Karthikeyan, University of South Carolina, Columbia, SC).

Gel Analysis of Fluorescent Fibronectins



Legend: Lane 1: Rhodamine laminin (Cat. LMN01); Lane 2: HiLyte488™ laminin (Cat. LMN02); Lane 3: Rhodamine fibronectin (Cat. # FNR01); Lane 4: HiLyte488™ fibronectin (Cat. # FNR02).

 

Providing highly active protein preparations and biochemical kits for over 20 years!

 

 

Cited in many peer reviewed articles

Some recent examples are:

 

Citation 1: 

Smith E.M. et al. 2015. Z-scan fluorescence profile deconvolution of cytosolic and membrane-associated protein populations.   

Anal. Biochem. 480, 11-20.

 

Citation 2: 

da Rocha-Azevedo B. et al. 2015. PDGF-stimulated dispersal of cell clusters and disruption of fibronectin matrix on three-dimensional collagen matrices requires matrix metalloproteinase-2. Mol. Biol. Cell. 26, 1098-1105.

 

Citation 3:

Torr E.E. et al. 2015. Myofibroblasts exhibit enhanced fibronectin assembly that is intrinsic to their contractile phenotype. J. Biol. Chem. 290, 6951-6961.

 

Some interesting applications:

 

App1: Measuring the effects of metalloproteinase on ECM using Rhodamine Fibronectin (Cat. # FNR01, see citation 2).

 

App2: In vitro invadopodia / podosome invasion assays (Cat. # FNR01, FNR02, LMN01, LMN02).

 

App3: Fibronectin assembly studies (Cat. FNR02, see citation 3).

 

App4: Measuring membrane dynamics in tissue culture using rhodamine fibronectin (Cat. # FNR01, see citation 1).

 

Also available are biotinylated fibronectin (Cat. # FNR03) and laminin (Cat. LMN03).

 

Have a question ?

 

Check out the About or FAQs tab in the ECM products section at  www.cytoskeleton.com/ecm or contact us directly at tservice@cytoskeleton.com .

 

 

Imagine the detail and clarity you can create by following live cells 

with these fluorescent probes...

Read More

Accurate and sensitive user-friendly formats with over 500 citations

Read More

Accurate One-Step Protein Assays

27/10/2017 11:35:08 AM

Advanced Protein Asay - Catalogue No -  ADV01

A highly sensitive assay for measuring low protein concentrations, protein in column fractions or measuring samples in SDS-PAGE loading buffer

proteinassay

Precision Red Protein Assay - Catalogue No. ADV02

An assay with a long linear range and good compatibility with many detergents, which makes it ideal for protein measurement in cell extracts, culture supernatants, purified protein and antibodies.

 

Top 5 Advantages over Bradford and Biuret methods.

1. One Step Operation 

 

2. Detergent Compatible

 

3. Rapid and Stable Color Development

 

4. Highly Sensitive Across a Wide Range of Proteins

 

5. Very Low Protein to Protein Variablity

For more information, contact us at info@huntingtree.co.nz 

 

Detection of ROS directly can be difficult, since the half-life of most free radicals is so short. A more reliable and well-published method is to detect the resulting damage caused by ROS. Proteins are a common target of ROS and provide an easy, sensitive method for testing oxidative stress levels.

In this edition we discuss various ways to test for specific protein damage markers. Continue reading for more details.

 

Protein Carbonyl Assays

The most common products of protein oxidation in biological samples are the carbonyl derivatives of proline, arginine, lysine, and threonine residues. Such derivaties are chemically stable and found universally across all proteins.

 

Many oxidative stress markers degrade over time, but protein carbonyl content can still be measured reliably even in samples frozen for 1-2 years. 

Our OxiSelect™ Protein Carbonyl Assays allow fast, reliable measurement of protein damage. A variety of formats is available including Western blot, fluorometric, spectrophotometric, and the most popular ELISA kit.

More information

Nitrotyrosine Assays

The presence of nitric oxide, via the intermediate reactive molecule peroxynitrite, most often results in nitration of tyrosine residues, i.e. formation of 3-Nitrotyrosine in proteins. The 3-Nitrotyrosine marker is extremely stable and easily measured in cell lysates, plasma, or serum.

 

Our Nitrotyrosine Assay Kits are available in two formats: Western blot and a 96-well competitive ELISA.

More information

 

 

Advanced Glycation End Product (AGE) Assays

AGE modification of proteins can contributeto the pathophysiology of aging and long-term complications of diabetes, atherosclerosis and renal failure.

Our range of AGE assays include an AGE ELISA Kit to broadly detect the formation of advanced glycation end products, as well as ELISA kits to detect specific AGE species: 

Methylglyoxal (MG)

The GOBlot Western Blot Processor is a reliable fluid delivery device for reproducibly probing blots and membranes with primary and secondary antibodies. It was developed after communication with hundreds of research scientists over the past two years. They required a cost-effective machine that would reproduce the hands-on method using a rocker platform and exchanging buffers manually.

GOBlot Western Blot Processor

The equipment is composed of valves, pumps, a motorized tilting platform and a control board that coordinates timing with the application of the correct solution to the membrane or blot. It can operate at room temperature or in a cold room*. All commonly used buffers are applicable, e.g. PBST and TBST. The purchase of the machine comes with a full warranty (parts & labor or replacement) for up to one year after the date of purchase.

* - Note cold room operation on order if applicable.

 

GOBlot™ Advantages

 

No Expensive Consumables and Recycles Your Primary Antibody

The GOBlot allows the operator to load up  their own membranes, add their blocking solution, primary and secondary antibodies.  Plus, the GOBlot makes it easy to recycle your primary antibody.

 

Choice of Four Routines

Choose from the four pre-loaded routines (See Manual).  Then press the start button and walk away.  Return after four hours to develop your blot using fluorescence, chemiluminescence or another method of detection. 

 

Modular and Economical

The machine is designed to be modular and economical so that you utilize multiple machines running at different times with different routines and different antibodies, in other words, it’s very flexible for multiple users. Also being modular means that you can add new units when the budget allows or your project demands it.

For more information on electro-transfer of proteins to membrances click here

PTM Toolkits

18/08/2017 2:28:50 PM

NEW Signal-Seeker publication in Bioscience Reports

  • Identification of 10 known PTMs from 3 distinct proteins
  • Novel identification of acetylated c-Fos
  • Investigated proteins from the membrane, cytoplasm, and nucleus
  • Targeted high, medium, and low abundance proteins
  • Tracked endogenous, physiologic changes in pY, SUMO 2/3, Ac, and Ub 

 ptmdata

Data from the manuscript... 

Click here to see the full article, or for more data generated using our Signal-Seeker PTM detection kits, see our validation data page or click on the links below

 

Signal Seeker Acetyl-Lysine Detection Tools

 

(Cat. # BK163) Signal-Seeker Acetyl-Lysine Detection Kits (30 Assays)  

 

(Cat. # AAC04-Beads)  Signal-Seeker Acetyl-Lysine Affinity Beads

 

(Cat. # BK 161) Signal-Seeker Ubiquitination Detection Kits (30 Assays)

 

(Cat. # UBA01-Beads) Signal-Seeker Ubiquitination Affinity Beads 

 

(Cat. # BK 162) Signal-Seeker SUMOylation 2/3 Detection Kits (30 Assays) 

 

(Cat. # ASM24-Beads) Signal-Seeker SUMOylation 2/3 Affinity Beads

 

(Cat. # BK 160) Signal-Seeker Phosphotyrosine Detection Kits (30 Assays)

 

(Cat. # APY03-Beads) Signal-Seeker Phosphotyrosine Affinity Beads

 

(Cat. # BLR01) Signal-Seeker: BLASTR Rapid Lysate Prep Kit

 

Signal-Seeker PTM unlabeled and HRP-labeled antibodies

 

Signal-Seeker Control Beads

Actin Ring-based Periodic Membrane Skeleton in Neuronal Axons 

 

Actin is an integral part of the neuronal cytoskeleton as it is involved in the regulation of neuronal polarization, cell morphology, the development of neuronal processes (i.e., growth cones with lamellipodial and filopodial extensions and dendritic spines), intracellular trafficking, and synaptic plasticity (dynamic changes in dendritic spine number and/or morphology). Actin's presence in growth cones and dendritic spines have garnered the attention of scientists for decades; however, actin is also found in neuronal axons, though its presence there has been described as the "black sheep of the neuronal actin family"... 

This is because the exact details of actin’s structure and role in the axon are unknown. Recently, significant advances have been made in unraveling the structure of axonal actin with the discovery of the periodic membrane skeleton (PMS) by nanoscopic microscopy5 (Fig. 1). This newsletter discusses the discovery, structure, and possible functions of the PMS in axons.

PMS Discovery and Structure

Discovered in 2013, the PMS is a type of cortical actin and the primary component of the actin cortex, a mixture of F-actin and actin binding proteins which supports eukaryotic cells’ plasma membrane and membrane-associated processes such as endo- and exocytosis and cell motility4,5. In neuronal axons, including the initial segment6, the PMS consists of short actin filaments bundled into evenly spaced rings that wrap around the circumference of the axon with a periodicity of 180-190 nanometers5-9 (Fig. 1). The short filaments are stabilized by an adducin cap which controls the diameter of actin rings and axons, as well as actin filament growth within the rings6,10. Adjacent actin rings are secured through cross-linkage by spectrin tetramers (bII in the axon proper and bIV in the axon initial segment)6,8,11.

Figure 1. Live cell imaging in neurons with STED microscopy and 100 nM SiR-actin. Upper left: Hippocampal rat neuron, 8 DIV. Upper right: Hippocampal rat neuron, 17 DIV. Bottom image: Rat cerebellar granule neuron, 21 DIV. All images courtesy of Elisa D'Este, MPI Biophysical Chemistry, Göttingen.
Figure 1. Live cell imaging in neurons with STED microscopy and 100 nM SiR-actin. Upper left: Hippocampal rat neuron, 8 DIV. Upper right: Hippocampal rat neuron, 17 DIV. Bottom image: Rat cerebellar granule neuron, 21 DIV. All images courtesy of Elisa D'Este, MPI Biophysical Chemistry, Göttingen.

The PMS was described for the first time in fixed mammalian neurons and brain tissue slices using stochastic optical reconstruction microscopy (STORM)5. Shortly thereafter, these findings were confirmed by others using STORM, stimulated emission depletion [STED] nanoscopy, and structured illumination microscopy [SIM] in fixed mammalian and non-mammalian neurons5,6,8,11,12, and most importantly, living mammalian neurons using either the F-actin live cell imaging probe SiR-actin7,10,13 (silicon rhodamine actin; Fig. 1) or exogenous expression of fluorescently-labeled bII spectrin8.

In cultured mammalian neurons, the PMS is established in the first few days of development in the axon proximal to the cell body before moving distally along the axon as the neuron develops5,7,8,11. In Drosophila primary neurons, development of the PMS begins within hours of plating11. Notably, the authors reported differences between the PMS of very young (hours to 2 days old) vs mature (>3 days old) Drosophila neurons. For instance, the PMS in young, growing axons, but not older axons, depends upon actin polymerization (i.e., nucleation factors)11.

The actin binding proteins involved in nucleation, assembly, and maintenance of the actin rings are relatively unknown. In Drosophila neurons, two nucleation factors, Arp2/3 and formin DAAM [disheveled-associated activator of morphogenesis], participate in PMS formation, likely nucleating the multiple short filaments that comprise the PMS11.

PMS Functions

Neuronal axons are the means by which neurons communicate and transport cargo anterogradely and retrogradely between the cell body and axon terminal. Maintaining healthy axons is necessary for normal brain function as axonal loss is permanent and underlies both normal aging deficits and various neurodegenerative disorders and diseases14. Functionally, the PMS has been hypothesized to serve as a scaffold for the axonal plasma membrane; spatially organize molecules important for axonal structure and action potential generation, such as ankyrin and sodium channels, respectively, into a periodic distribution in axons5,8; assist in the docking of motor-associated cargo vesicles, especially along the distal part of the axon4,9,15; and organize transmembrane proteins along the axon11,15-18.

Actual functional data are sparse; however, a recent report using Drosophila primary neurons fixed and stained with SiR-actin concluded that the PMS is important in maintaining axon integrity by stimulating the polymerization of axonal microtubules (MTs), another primary cytoskeletal component of axons11. Cytochalasin D-induced PMS disassembly resulted in breaks in MT bundles, reduced MT polymerization, and reduced axon numbers11. Other MT-associated functional roles for PMS might involve assembling MTs into bundles19-21; serving as anchors for the minus end of MTs22,23; and supporting dynein-mediated sliding of MTs and transport24.

Summary

The recent discovery of actin rings that comprise a sub-membranous lattice in neuronal axons offers an exciting opportunity to better understand the role of axonal actin. Moreover, actin rings have also been described in the nodes of Ranvier in the peripheral nervous system and in at least some dendrites and dendritic spines7,25. Similar to actin rings, actin waves and trails are other actin structures that have received little attention4. Without super-resolution microscopy (e.g., STORM, STED, SIM) and the development of live cell imaging probes such as SiR-actin, the existence and composition of the PMS would remain undiscovered. To help scientists further discover and define the composition, assembly, maintenance, and function of actin and other cytoskeletal structures, Cytoskeleton, Inc., provides SiR live cell imaging probes for F-actin, microtubules, DNA, and lysosomes, along with Acti-stain phalloidins for use in fixed cells.

 


Related Products & Resources

Spirochrome™ Live Cell Imaging Probes

SiR700-Actin Kit (Cat. # CY-SC013)

SiR-Actin Kit (Cat. # CY-SC001)

SiR700-Tubulin Kit (Cat. # CY-SC014)

SiR-Tubulin Kit (Cat. # CY-SC002)

SiR-Lysosome Kit (Cat. # CY-SC012)

SiR700-Lysosome Kit (Cat. # CY-SC016)

SiR-DNA Kit (Cat. # CY-SC007)

SiR700-DNA Kit (Cat. # CY-SC015)

Cytoskeleton Kit, SiR-Actin and SiR-Tubulin (Cat. # CY-SC006)

Actin Proteins

Actin Protein (rhodamine): human platelet (Cat. # APHR)

Actin Protein (rhodamine): rabbit skeletal muscle (Cat. # AR05)

Acti-stain Phalloidins

Acti-stain™ 488 Phalloidin (Cat. # PHDG1)

Acti-stain™ 555 Phalloidin (Cat. # PHDH1)

Acti-stain™ 670 Phalloidin (Cat. # PHDN1)

Phalloidin (rhodamine) (Cat. # PHDR1)

Actin Activation Assay Biochem Kit

G-Actin/F-Actin In Vivo Assay Biochem Kit (Cat. # BK037)


References

  1. Luo L. 2002. Actin cytoskeleton  regulation in neuronal morphogenesis and structural plasticity. Annu. Rev. Cell Dev. Biol. 18, 601-635.
  2. Cingolani L.A. and Goda Y. 2008. Actin in action: The interplay between the actin cytoskeleton and synaptic efficacy. Nat. Rev. Neurosci. 9, 344-356.
  3. Stiess M. and Bradke F. 2011. Neuronal polarization: The cytoskeleton leads the way. Dev. Neurobiol. 71, 430-444.
  4. Roy S. 2016. Waves, rings, and trails: The scenic landscape of axonal actin. J. Cell Biol. 212, 131-134.
  5. Xu K. et al. 2013. Actin, spectrin, and associated proteins form a periodic cytoskeletal structure in axons. Science339, 452-456.
  6. Leterrier C. et al. 2015. Nanoscale architecture of the axon initial segment reveals an organized and robust scaffold. Cell Rep13, 2781-2793.
  7. D’Este E. et al. 2015. STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons. Cell Rep. 10, 1246-1251.
  8. Zhong G. et al. 2014. Developmental mechanism of the periodic membrane skeleton in axons. eLife. 3, e04581.
  9. Leite S.C. and Sousa M.M. 2016. The neuronal and actin commitment: Why do neurons need rings? Cytoskeleton.73, 424-434.
  10. Leite S.C. et al. 2016. The actin-binding protein a-adducin is required for maintaining axon diameter. Cell Rep. 15, 490-498.
  11. Qu Y. et al. 2017. Periodic actin structures in neuronal axons are required to maintain microtubules. Mol. Biol. Cell.28, 296-308.
  12. He J. et al. 2016. Prevalent presence of periodic actin-spectrin-based membrane skeleton in a broad range of neuronal cell types and animal species. Proc. Natl. Acad. Sci. U.S.A. 113, 6029-6034.
  13. Lukinavicius G. et al. 2014. Fluorogenic probes for live-cell imaging of the cytoskeleton. Nat. Methods. 11, 731-733.
  14. Adalbert R. and Coleman M.P. 2012. Axon pathology in age-related neurodegenerative disorders. Neuropathol. Appl. Neurobiol. 39, 90-108.
  15. Gallo G. 2013. More than one ring to bind them all: Recent insights into the structure of the axon. Dev. Neurobiol.73, 799-805.
  16. Zhang Y. et al. 2016. Axon membrane skeleton structure is optimized for coordinated sodium propagation. arXiv.1602.06348.
  17. Albrecht D. et al. 2016. Nanoscopic compartmentalization of membrane protein motion at the axon initial segment. J. Cell Biol215, 37-46.
  18. Machnicka B. et al. 2014. Spectrins: a structural platform for stabilization and activation of membrane channels, receptors and transporters. Biochim. Biophys. Acta. 1838, 620-634.
  19. Prokop A. et al. 2013. Using fly genetics to dissect the cytoskeletal machinery of neurons during axonal growth and maintenance. J. Cell Sci. 126, 2331-2341.
  20. Sanchez-Soriano N. et al. 2009. Mouse ACF7 and Drosophila Short stop modulate filopodia formation and microtubule organization during neuronal growth. J. Cell Sci. 122, 2534-2542.
  21. Alves-Silva J. et al. 2012. Spectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent +TIPs (tip interaction proteins). J. Neurosci. 32, 9143-9158.
  22. Nashchekin D. et al. 2016. Patronin/Shot cortical foci assemble the noncentrosomal microtubule array that specifies the Drosophila anterior-posterior axis. Dev. Cell. 38, 61-72.
  23. Ning W. et al. 2016. The CAMSAP3-ACF7 complex couples noncentrosomal microtubules with actin filaments to coordinate their dynamics. Dev. Cell. 39, 61-74.
  24. Myers K.A. et al. 2006. Microtubule transport in the axon: Re-thinking a potential role for the actin cytoskeleton. Neuroscientist. 12, 107-118.
  25. Bar J. et al. 2016. Periodic F-actin structures shape the neck of dendritic spines. Sci. Rep. 6, 37136.

 

 

 

it’s with pleasure to inform you that LDN has launched today its latest development – the first commercial immunoassay worldwide for the quantitative determination of

3-Methoxytyramine (3-MT)

in human plasma.

The determination of normetanephrine and metanephrine in plasma is a sensitive test for diagnosing phaechromocytoma and paragangliomas (PPGLs) – but it does not provide the possibility to detect dopamine-producing tumours. Dopamine and especially its O-methylated metabolite 3-MT are reliable biomarkers for the detection of malignant PPGLs.

This new assay compares with excellence to the LC-MS/MS method (please refer to the flyer attached) and offers an accurate, selective and sensitive measurement of 3-MT.

The assay itself (catalogue number: BA R-8800; please refer to the instructions for use in the attachment) uses an elegant extraction procedure followed by a competitive RIA.

 

With this new assay LDN is now able to offer the most comprehensive panel worldwide for the measurement of PPGL-relevant parameters including:

  • Urinary Metanephrine/Normetanephrine (free and total; ELISA&RIA)
  • Plasma Metanephrine/Normetanephrine (ELISA&RIA)
  • Catecholamines in plasma and urine (ELISA&RIA)
  • Chromogranin A (ELISA)

 

For more information, please  contact us at info@huntingtree.co.nz 

 

All our Lightning-Link® antibody labeling kits have been carefully designed to guarantee successful conjugation provided the protocol is followed correctly, but for extra peace of mind we have developed our Conjugate Check&Go! product range. This includes HRP Check&Go!, a dipstick lateral flow assay for confirming the successful conjugation of HRP to an IgG antibody.

 

The HRP Check&Go! process. The HRP-antibody conjugate is run on the Protein A/G Strip. The conjugate binds the Protein A and Protein G which are concentrated at the Test line; following the addition of the HRP detection solution, a visible line on the strip indicates successful conjugation.

hrp_checkngo
The HRP Check&Go! Kit is compatible with IgG antibodies from multiple species, provided they have affinity for either Protein A or G. The entire process takes just 25 minutes, and requires only small volumes of diluted conjugate.

Quantify GFP or RFP by Simple ELISA

14/06/2017 10:37:56 AM

Quantify GFP or RFP by Simple ELISA          
Now you don't need a flow cytometer to quantify GFP or RFP. Our easy-to-use ELISA Kits allow you to quantify GFP or RFP right on your benchtop using a standard microplate reader, with superior sensitivity compared to direct fluorescence measurement.

  • The GFP ELISA Kit is designed to accurately measure enhanced variants of Aequora victoria GFP, including EGFP, ECFP, EBFP and EYFP.  
  • The RFP ELISA Kit quantifies a variety of red fluorescent protein variants including TagRFP, TurboRFP, DsRed, mCherry, mKate, mOrange, mPlum, mRuby, mStrawberry, and tdTomato 

gfp

Accurate and sensitive user-friendly formats with over 500 citations

 

rac1

Swiss 3T3 fibroblasts were serum starved for 24h, then either treated with 10 ng/ml of EGF for 2 min (Lanes 4 & 5) or left untreated (Lanes 2 & 3).  Rac1 activation was measured using the Rac1 Activation pull-down assay (Cat. # BK035) (Lanes 2-5).  Lane 1 is 20 ng of recombinant Rac1-His protein.

rhoaSwiss 3T3 fibroblasts were treated with 1 μg/ml CN04 for the indicated times and cell lysates were subjected to G-LISA activation assays for RhoA (Cat.# BK124), Rac1 (Cat.# BK128), and Cdc42 (Cat.# BK127).

 
 

Applications for pull-downs

 

Measure K-Ras, N-Ras, H-Ras, RhoC, Rac3 isoforms

  • Active levels of multiple Ras- and Rho-subfamily GTPase isoforms can be detected

Complement G-LISA findings

  • Use two different, validated techniques to quantitate active GTPase level.

 

Applications for G-LISAs

 

Measure active GTPases in 3-D cell cultures

Measure active GTPases in primary cell lysates

  • Measure active GTPases in as little as 5 µg of total cell extract.

Increase accuracy of active GTPase quantitations

  • Quantitative numerical output in OD490nm units.

Measure active GTPases in tissue lysates

  • Use as little as 1 mm3 of tissue for extraction.

 

Cited in hundreds of peer- reviewed articles

3-D Cell Culture and G-LISAs: 

 

Ponik S.M. et al. 2013. RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis. Mol. Biol. Cell. 24, 1688-1699. 

 

Petroll W.M. et al. 2008. Dynamic assessment of fibroblast mechanical activity during Rac-induced cell spreading in 3-D culture. J. Cell Physiol. 217, 162-171. 

 

Keely P.J. et al. 2007. Investigating integrin regulation and signaling events in three-dimensional systems. Methods Enzymol. 426, 27-45. 

 

 

Primary Cells and G-LISAs: 

 

Valdez C.M. et al. 2016. The Rac-GAP alpha2-chimaerin regulates hippocampal dendrite and spine morphogenesis.  Mol. Cell. Neurosci. 75, 14-26. 


Nini L. and Dagino L. 2010. Accurate and reproducible measurements of RhoA activation in small samples of primary cells. Anal. Biochem. 398, 135-137. 


Flaiz C. et al. 2009. PAK kinase regulates Rac GTPase and is a potential target in human schwannomas. Exp. Neurol. 218, 137-144. 

 

Increased Accuracy and G-LISAs:

 

Oliver A.W. et al. 2011. The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42. Br. J. Cancer. 104, 324 - 331. 

 

GTPase Isoforms and Pull-downs:

 

Tabibian J.H. et al. 2014. Cholangiocyte senescence by way of N-Ras activation is a characteristic of primary sclerosing cholangitis. Hepatology. 59, 2263-2275. 


Kusuhara S. et al. 2012. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothe­lial cells. PLoS ONE. 9:e45858. 

 

Pull-downs Complementing G-LISAs:

 

Tanaka T. et al. 2010. Monocyte chemoattractant protein-1/CC chemokine ligand 2 enhances apoptotic cell removal by macrophages through Rac1 activation. Biochem. Biophys. Res. Commun. 399, 677-682. 

 

Schlegel N. and Waschke J. 2009. VASP is involved in cAMP-mediated Rac 1 activation in microvascular endothelial cells. Am. J. Physiol. Cell Physiol. 296, C453-C462. 

 

Lai F.P.L. et al. 2009. Cortactin Promotes Migration and Platelet-derived Growth Factor-induced Actin Reorganization by Signaling to Rho-GTPases. Mol. Biol. Cell. 20, 3209-3223. 

 

G-LISA Activation Assays

Cat. #

Amount (assays)

BUNDLE RhoA / Rac1 / Cdc42 Activation Assay G-LISA Kits

BK135

24 each

Arf1 Activation Assay G-LISA Kit;  Colorimetric

BK132

96

Arf6 Activation Assay G-LISA Kit;  Colorimetric

BK133

96

Cdc42 Activation Assay G-LISA Kit;  Colorimetric

BK127

96

Rac1,2,3 Activation Assay G-LISA Kit;  Colorimetric

BK125

96

Rac1 Activation Assay G-LISA Kit;  Colorimetric

BK128

96

Rac1 Activation Assay G-LISA Kit;  Luminescence

BK126

96

RalA Activation Assay G-LISA Kit;  Colorimetric

BK129

96

Ras Activation Assay G-LISA Kit, Colorimetric

 BK131

96

RhoA Activation Assay G-LISA Kit;  Colorimetric

BK124

96

RhoA Activation Assay G-LISA Kit;  Luminescence

BK121

96

     

Pull-down Activation Assays

   

COMBO RhoA/Rac1/Cdc42 Pull-down Activation Assay

BK030

10 each

Arf1 Pull-down Activation Assay

BK032-S

20

Arf6 Pull-down Activation Assay

BK033-S

20

Cdc42 Pull-down Activation Assay

BK034

50

Rac1 Pull-down Activation Assay

BK035

50

RalA Pull-down Activation Assay

BK040

50

Ras Pull-down Activation Assay

BK008

50

RhoA Pull-down Activation Assay

BK036

80

G-switch Activators & Inhibitors

Target endogenous Rho family proteins and pathways

Read More

Biorbyt Focus CD3

22/05/2017 11:27:04 AM

CD3 - Cluster of differentiation 3

CD3 antigen can be found bound to the membranes of all mature T-cells and in virtually no other cell type. This high specificity, combined with its presence at all stages of T-cell development makes it a useful T-cell marker for IHC tissue sections. The antigen is also present in almost all T-cell lymphomas and leukaemias, allowing their differentiation from similar B-cell and myeloid neoplasms.

CD3 Reagents

Biorbyt offers you a superb choice of CD3 Antibodies and Proteins for your work;

  • Over 270 products to choose from
  • Monoclonal antibodies to CD3 and CD3 Epsilon
  • Polyclonal antibodies to CD3 and CD3 Epsilon
  • Human, Mouse and Monkey CD3 proteins
  • Heterodimer and biotinylated CD3E, CD3D & CD3G proteins

We are one of the only sources for Biotinylated Human CD3 epsilon and Human CD3E & CD3D heterodimer protein. We also offer the hard to find Mouse CD3 epsilon protein.

 

Why not look through and refine your search for our whole CD3 range.

cd3Immunohistochemical staining of rat skin tissue using anti-CD3 (2.5 ug/ml)

 

Have you seen our ELISA kits?

We now have over 7000 great quality ELISA kits for you to choose from.

  • Great species choice
  • Great target choice
  • Matched pairs for your own development projects

 

 

Having trouble with your application?….

Our dedicated Technical Support team are here to help you whatever the time of day.  We have experts in most applications just waiting to talk to you and make your research shine.  

You can call us: +44 (0)1223 859 535

                          +1 (415) 906 5211

Email us: support@biorbyt.com

                      or

Fill in our Technical Support form

You'll always receive an answer within 12 hours - WE LOVE TO HELP YOU

Introduction

The small GTPase ADP-ribosylation factor 6 (Arf6) belongs to the Arf subfamily of Ras superfamily GTPases. Of the three classes of Arf GTPases, Arf6 is the only member of class III and uniquely localizes to the plasma membrane and endosomes, positioning it to regulate cellular processes dependent upon dynamic changes in the actin cytoskeleton, including endocytosis, exocytosis, trafficking/recycling of membrane-localized proteins, and membrane protrusions (e.g., ruffles). These cellular functions underlie physiological and pathological cell motility and intracellular trafficking. Arf6 cycles between an inactive, GDP-bound state and an active, GTP-bound state to act as a molecular switch in the cellular processes listed above. Activation of Arf6 by exchange of GDP for GTP is mediated by guanine exchange factors (GEFs) while inactivation by GTP hydrolysis is mediated by GTPase activating proteins (GAPs)1-3. In this newsletter, we discuss the mechanistic roles Arf6 and its GEFs have in cancer cell invasion and metastasis.

Arf6 GEFs and Cancer

Cancer cell invasion and metastasis require dynamic changes in the actin cytoskeleton. Recent work demonstrates that Arf6 has an essential role in tumor angiogenesis and growth, as well as cancer cell invasion and metastasis4-8. Moreover, Arf6 GEFs such as BRAG2/GEP100, cytohesin3/Grp1, and EFA6 are also involved in cancer progression5,7,8. Prevention of Arf6 activity/cycling inhibits cancer progression6,7,9.

Of the fourteen known Arf GEFs, eight target Arf6, and of those, five are Arf6-specific. Arf6 GEFs are activated directly through binding with the pleckstrin homology (PH) domain and recruited to the plasma membrane by binding to phosphoinositides produced by phosphatidylinositol 3-kinase (PI3K), as well as a variety of ligand-activated cell surface receptors. There the GEFs' catalytic Sec7 domain activates membrane-localized Arf6. BRAG2/GEP100 and EFA6A-D activate only Arf6 while cytohesin1, cytohesin2/ARNO, and cytohesin3/Grp1 target Arf6, among other Arfs1-3.

GEP100, the best-characterized Arf6 GEF, promotes the invasion of breast cancer, melanoma, and lung adenocarcinoma cells5,6,10,11. In response to epidermal growth factor (EGF) stimulation, GEP100 directly binds the ligand-occupied EGF receptor (EGFR) and activates Arf6 at the plasma membrane5 (Fig. 1). In turn, GTP-bound Arf6 recruits and activates AMAP1, a downstream effector, which mediates invadopodia formation, the initial step in cancer cell invasion10. Activated Her2, human epidermal growth factor 2, also stimulates GEP100-mediated Arf6 activation, which positively regulates cancer cell invasion11. The vascular endothelial growth factor (VEGF) also activates Arf6 via GEP100, stimulating angiogenesis in endothelial cells12. GEP100-mediated activation of Arf6 also stimulates cancer cell invasion and metastasis via disassembly of E-cadherin-mediated cell-cell adhesion10 (Fig. 1). In addition, the Wnt family member 5A (WNT5A) also activates Arf6 through GEP100 in melanoma cells. WNT5A-mediated activation of Arf6 through GEP100 disassembles the b-catenin/cadherin complex. Unbound b-catenin promotes tumor cell invasion and metastasis through b-catenin-mediated transcription and disruption of cell-to-cell adhesions (i.e., adherens junctions)6 (Fig. 1). These effects are prevented in the presence of SecinH3, a small-molecule inhibitor of GEP100 and the cytohesin family of Arf GEFs6.

arf6gef

Within the EFA6 family of Arf6 GEFs, a positive or negative regulatory effect is possible, depending on the particular EFA6 GEF isoform and type of cancer. For example, EFA6A positively regulates glioma cell invasion13, while EFA6B antagonizes breast cancer cell invasion14. The EFA6A-mediated effects utilize the extracellular signal-regulated kinase (ERK) signaling cascade13, which complements an earlier finding that Arf6-induced melanoma cell invasion requires ERK signaling15. Complicating matters is the capacity of EFA6A, B, and C isoforms to positively regulate cancer cell invasion, metastasis, and drug resistance8.


Grp1-mediated activation of Arf6 regulates hepatocyte growth factor (HGF)-dependent tumor angiogenesis via an Arf6-stimulated increase in b1 integrin recycling from the endosomes to the plasma membrane7 (Fig. 1). b1 integrin recycling to the plasma membrane is necessary for anchoring of cancer cells to allow maturation of newly formed blood vessels. SecinH3 inhibits these effects7. Grp1 has also been reported to control breast cancer cell migration16.

Clinical Significance: Small molecule inhibitors of Arf6

Inhibition of cytohesins and GEP100 is accomplished with the small-molecule Arf GEF inhibitor SecinH3, as discussed above. In animal models, SecinH3 inhibits glioma cell metastasis and angiogenesis of melanoma and lung carcinoma tumors6,7; however, treated animals develop hepatic insulin resistance17. Another molecule is PIT-1 which inhibits phosphatidylinositol-3,4,5-trisphosphate (PIP3), the lipid product of PI3K and regulator of the Akt cell survival and growth signaling pathway. PIT-1 prevents PIP3 binding to the PH domains of ARNO and Grp1 which results in inhibition of lamellipodia formation and breast cancer cell migration1,2,16. In addition, melanoma tumor angiogenesis and metastasis are inhibited. Additional work is required to differentiate the contributions of ARNO and Grp1 to tumor invasion/metastasis and determine if one or both GEFs are targeted in PIT-1’s anti-cancer effects.

Conclusions

Recent studies of the Arf6 GTPase and its GEFs demonstrate that these proteins have a clear role in cancer cell invasion and metastasis and tumor angiogenesis. Moreover, some findings suggest that specific cancers are associated with certain GEFs. This observation offers the possibility of very selective, novel anti-cancer treatments targeting Arf6 and/or its GEFs. To assist scientists in these studies, Cytoskeleton Inc., offers Arf6 activation assay kits along with kits for many other Ras superfamily GTPases. The activation assays come in two convenient, easy-to-use formats, the effector bead pull-down assay and the ELISA-based G-LISA assay. In addition, we offer GEF and GAP exchange assay kits and reagents for monitoring changes in F-actin in fixed and live cells. To learn more about these and other tools for measuring GTPase-mediated, dynamic changes in the cytoskeleton, please contact one of our technical support scientists at tservice@cytoskeleton.com

Live Cell Probes

18/04/2017 1:34:37 PM

Imagine the detail and clarity you can create by following live cells 

with these fluorescent probes...

Read More

The use of activated magnetic particles makes immunomagnetic separation easier, faster and more effective. It offers a convenient way to conjugate any desired ligands to a magnetic particle surface and provides an extremely stable linkage between the ligand and the solid matrix.

Absolut Mag™ Activated Magnetic Particles are pre-activated with high density functional groups and can therefore be used for direct coupling of proteins, peptides, nucleic acids or other ligands without further surface activation and lengthy reaction schemes, ensuring rapid and efficient binding of your target molecules under conditions causing minimal stress.

cdmagneticsep

 

Product Name

Target Ligand

Absolute Mag™ NHS-activated Magnetic Particles

Primary amine group-containing ligands.

Absolute Mag™ Tosyl Magnetic Particles

Direct covalent binding of ligands via primary amine- or sulphydryl groups.

Absolute Mag™ Epoxy Magnetic Particles

Amine, sulfhydryl, or hydroxyl group-containing ligands.

Absolut Mag™ Maleimide Activated Magnetic Particles

Thiol group-containing ligands.

 

 

APPLICATIONS

 

Cell separation

 

Immunoassays and immunodiagnostics

 

Immunoprecipitation

 

Affinity purification: Antibody purification, Protein/peptide purification, DNA/RNA purification, etc.

 

 

FEATURES

 

No further surface activation required

 

Stable covalent bond with minimal ligand
leakage

 

Low non-specific binding

 

Mild reaction conditions

 

Ensure the activity of coupled ligands

 

Easy to use: simple mix-and-go format

Absolut Mag™ Activated Magnetic Particles Ordering Information

Cat. No.

Description

Particle Size

Package Size

WHM-X019

NHS-activated Magnetic Particles

50 nm

10 mg

WHM-X021

NHS-activated Magnetic Particles

100 nm

10 mg

WHM-X022

NHS-activated Magnetic Particles

150 nm

10 mg

WHM-X023

NHS-activated Magnetic Particles

200 nm

10 mg

WHM-X024

NHS-activated Magnetic Particles

500 nm

10 mg

WHM-X025

NHS-activated Magnetic Particles

1 µm

10 mg

WHM-C299

NHS-activated Magnetic Particles, PVA coated

0.5-1 µm

2 mL

WHM-C300

NHS-activated Magnetic Particles, PVA coated

1-3 µm

2 mL

WHM-Q009

Epoxy Magnetic Particles, Silica Matrix, Embedded

1-2 µm

10 mL

WHM-Q010

Epoxy Magnetic Particles, Silica Matrix, Embedded

2-3 µm

10 mL

WHM-Q011

Epoxy Magnetic Particles, Silica Matrix, Embedded

3-4 µm

10 mL

WHM-Q012

Epoxy Magnetic Particles, Silica Matrix, Embedded

4-5 µm

10 mL

WHM-Q051

Epoxy Magnetic Particles, UF Matrix

1-2 µm

10 mL

WHM-Q075

Epoxy Magnetic Particles

0.5-1.0 µm

10 mL

WHM-Q116

Epoxy Magnetic Particles, γ-Fe2O3

0.1-1 µm

10 mL

WHM-C307

Epoxy Magnetic Particles, PVA coated

0.5-1 µm

10 mL

WHM-C308

Epoxy Magnetic Particles, PVA coated

1-3 µm

10 mL

WHM-K031

Maleimide Activated Magnetic Particles Conjugation Kit

50 nm

10 mg

WHM-K032

Maleimide Activated Magnetic Particles Conjugation Kit

75 nm

10 mg

WHM-K033

Maleimide Activated Magnetic Particles Conjugation Kit

100 nm

10 mg

WHM-K034

Maleimide Activated Magnetic Particles Conjugation Kit

150 nm

10 mg

WHM-K035

Maleimide Activated Magnetic Particles Conjugation Kit

200 nm

10 mg

WHM-K036

Maleimide Activated Magnetic Particles Conjugation Kit

500 nm

10 mg

WHM-K037

Maleimide Activated Magnetic Particles Conjugation Kit

1 µm

10 mg

WHM-L081

Tosyl Magnetic Particles

1 µm

2 mL

WHM-L075

Tosyl Magnetic Particles

2.8 µm

2 mL

WHM-L030

Tosyl Magnetic Particles

4.5 µm

5 mL

Please feel free to contact us at info@huntingtree.co.nz for any questions. We are more than happy to have a chance to work with you.

Stable Phosphate Detection System

22/02/2017 4:23:55 PM

Methods for measuring inorganic phosphate (Pi) using dyes such as Malachite Green are well known, however the dye-Pi complexes are prone to precipitation and the reagents are very acidic which can lead to non-enzymatic hydrolysis of many phosphorylated substrates.

At the heart of the Innova Biosciences' range of phosphate detection products is PiColorLockTM a unique phosphate detection reagent that overcomes these common issues. The formulation of PiColorLockTM affords enhanced assay linearity, dynamic range and color stability, and it is also possible to work with unstable substrates that give high non-enzymatic background signals with other acidic dye-based detection reagents.

PiColorLockTM can be purchased singularly or as part of our ATPase and GTPase assay kits.

For more information about our range of phosphate detection products, take a look at our video below:

https://youtu.be/KoqDgj2GDQc 

For more information, or for a quote, please mail us at info@huntingtree.co.nz 

 

picolourlock
The PiColorLockTM reagent is often used with unstable substrates (e.g. ATP, GTP) that give rise to non-enzymatic background drift with time. The unique stabilizer, provided in the PiColorLockTM phosphate detection system, blocks this non-enzymatic breakdown generating a stable low background. The stable signal can therefore be read up to several hours after the reaction has ended.

Recently, Jeganathan et al. examined the role of intersectin-1s (ITSN-1s) in lung cancer proliferation, migration, and metastasis. These cellular processes require actin cytoskeleton re-arrangement typically regulated by RhoA, Rac1, and/or Cdc42 GTPases. ITSN-1s is a multi-domain adaptor protein linking cell surface receptors to intracellular signaling cascades. ITSN-1s's expression levels are reduced in human lung cancer cells and tissues. Among many findings, the authors report that ITSN-1s down-regulates the epidermal growth factor receptor kinase substrate 8 (Eps8) via ubiquitination and degradation, which in turn decreases the Eps8 and Ras/Rac1 guanine exchange factor mSos1 complex. The Eps8/mSos1 complex activates Rac1 which mediates the subsequent actin cytoskeletal re-arrangements necessary for cancer cell migration and metastasis. Restoration of normal ITSN-1s levels decreases Rac1 activation, increases RhoA activation (leaving Cdc42 activity unaffected), and results in a cytoskeletal network unfavorable to cancer cell migration and metastasis. Cytoskeleton's RhoA, Rac1, and Cdc42 pull-down activation assays (Cat.# BK036, BK035, and BK034, respectively) were essential for the quantification of RhoA, Rac1, and Cdc42 activities across different levels of ITSN-1s expression. These results suggest that ITSN-1s could serve not only as a novel therapeutic target, but also a prognostic and therapeutic response indicator for lung cancer (and maybe others).

 

Read more... 

 

Link to citation: 


Jeganathan N. et al. 2016. Rac1-mediated cytoskeleton rearrangements induced by intersectin-1s deficiency promotes lung cancer cell proliferation, migration and metastasis. Mol. Cancer. 15,59. 

 

Products used in this citation:

RhoA Pull-Down Activation Assay Biochem Kit (bead pull-down format) - 80 Assays
(Cat. # BK036) 

Rac1 Pull-Down Activation Assay Biochem Kit (bead pull-down format) - 50 Assays
(Cat. # BK035) 

Cdc42 Pull-Down Activation Assay Biochem Kit (bead pull-down format) - 50 Assays
(Cat. # BK034) 

 Serum proteins comprise several groups of blood-based proteins that serve a variety of functions, and many can serve as potential disease markers. 


We offer a large portfolio of ELISA kits and similar assays for the quantitation of various serum proteins. Read on for further details.

 

Albumin Assays 

Albumins make up more than half of all protein found in the bloodstream. We offer two kits for the quantitation of albumins in blood. Both assays are quantified in a standard colorimetric plate reader:

  • Our BCG Albumin Assay Kit uses a proprietary dye formulation that produces a color complex when bound to albumin. The color formation takes only about 5 minutes.
  • Our Human Albumin ELISA Kit is an immunoassay that can detect albumin levels as low as 5 ng/m

 


Globulin Assays 

Globulins make up another significant portion of the protein content in blood, but may also be found in cells. Each one of our Globulin ELISA Kits quantifies a specific globular protein.



Cardiac Biomarkers

Cardiac markers are useful for assessing damage following various cardiac events including myocardial infarctions. Our ELISA Kits quantify these markers in serum, plasma, or cell lysates.

 

Clotting Factors 

Clotting (coagulation) factors work in conjunction with platelets to ensure the blood clots properly and to control bleeding. 

  • Our D-dimer ELISA Kit provides a convenient way to measure D-dimer, which can serve as a marker for deep vein thrombosis and pulmonary embolism.
  • Our Plasminogen ELISA Kit measures the concentration of plasminogen, a precursor to the active form plasmin that digests fibrin in the clotting cascade.

 

 

3 New P450 Catalytic systems

31/10/2016 12:24:37 PM

We are pleased to announce that we've added three new P450 catalytic systems to our CypExpress™ line.  CypExpress™ 1A1, CypExpress™ 2B6, and CypExpress™ 2E1 are now available.

CypExpress™ is a stable dry biocatalytic system containing both an individual recombinant human P450 and recombinant oxidoreductase along with cofactors and antioxidant systems produced in a eukaryotic expression system.

The identification and charachterization of metabolites is a critical step in the process of bringing any new chemical entity to market. Until now, the synthesis and purification of several milligrams of a given P450 metabolite required expensive E. coli recombinant enzymes or liver microsomes which can yield complex product mixtures and involve difficult isolation procedures.

CypExpress™ provides for robust metabolite identification and scale-up production while reducing the time and expense involved. 

Advantages

  • Coexpressed unmodified full-length human recombinant CYP and oxidoreductase
  • Provided as a dry powder that is stable at room temperature or frozen
  • Reactions can be performed in Tris or Phosphate buffers
  • Can be pelleted and used for multiple reaction cycles
  • Provides clean HPLC profiles for easy metabolite ID and purification
  • Produces much more metabolite faster and at a lower cost

 

Products

    CypExpress™ 1A1  NEW!

    CypExpress™ 1A2

    CypExpress™ 3A4

    CypExpress™ 2B6  NEW!

    CypExpress™ 2C9

    CypExpress™ 2C19

    CypExpress™ 2D6

    CypExpress™ 2E1  NEW!

for a full quote - please e-mail us at info@huntingtree.co.nz 

Magnetic Antibody Purification

22/09/2016 5:41:28 PM

 

 innovalogo

Our AbPure™ Magnetic Purification System is here!

Innova Biosciences announce that they have expanded their range of purification kits - their AbPure™ Magnetic Purification System is a universal purification solution that has been specifically developed for the purification of low antibody amounts.
The kit works by capturing the antibody on Protein A magnetic beads. Unwanted substances can then be removed from the antibody storage buffer by collecting the beads in a magnetic stand, washing them, and then eluting the antibody.

abpureprocess

 

The AbPure™ Magnetic Purification System is compatiblewith a wide range of labeling technologies, including  Lig htning-Link®, Thunder-Link®, InnovaCoat® and Latex conjugation kits.

 

Quick and easy to use

Purify from 20 - 200ug of antibody

High recovery: 70-90%

Flexible and reliable

 

The product code for this kit is 265-0200,  The accompanying magnetic stand has product code 265-1006 .

If you have any questions please don't hesitate to get in touch!

 

 

 

 

ChromoTek nano-traps are the fastest, simplest, cleanest solution for purifying GFP & RFP tagged proteins 

 

abinGFP RFP purification

More than 700 peer reviewed publications have cited use of the GFP-trap.
Try it yourself and find out just why it's so popular!

 

Agarose Bead GFP-Trap (ABIN509397)

Magnetic Bead GFP-Trap (ABIN509401)

Magnetic & Agarose Bead GFP-Trap (ABIN1889488)

Agarose Bead RFP-Trap (ABIN509408)

Magnetic Bead RFP-Trap (ABIN509412)

Magnetic & Agarose Bead RFP-Trap (ABIN2452223)

 

ChromoTek nanobodies are small, recombinant antigen binding fragments derived from the VHH domain of the alpaca antibody.

Bound to agarose or magnetic beads, nanobodies form a nano-trap - an elegantly simple reagent that makes immunoprecipitation fast, clean, and easy.

 

Nano-traps like the GFP-trap and RFP-trap are better tools for IP than traditional monoclonal or polyclonal antibodies, because they are:

  • Cleaner: Heavy and light chain contamination are always problematic concerns in an IP - nanotraps have neither a heavy nor a light chain, and contain only the fragment of the antibody necessary for antigen binding
  • Faster:  A nano-trap immunoprecipitation can be completed in just one hour (less than one-third the time required for a traditional antibody IP)
  • More Consistent & Reproducible: Recombinantly produced nano-traps don't suffer from cell line drift, or the batch specific variance issues commonly associated with monoclonal or polyclonal antibodies
  • Better Binders: Nano-traps have exceptionally high affinity constants. The RFP-trap and GFP-trap boast respective 5 nanomolar and 1 picomolar dissociation constants!
  • More Efficient: Smaller size and higher affinity means that significantly more protein can be bound per uL of GFP or RFP-trap slurry (when compared to conventional mono or polyclonal antibodies)

 

ne

 

 
Antigen
25-OH VitD3-BSA Conjugated
Cat. No. DAG03219
This is the first commercially available raw material for 25-OH VitaminD3 ELISA analysis. The heptan 25-OH VitD3 is conjugated with the carrier bovine serum albumin (BSA).
Matched Antibody
Monoclonal Antibody We also prepare the matched antibody for manufacturers who want to make ELISA kits on their own or researchers who want to conduct ELISA experiments with highly sensitive, specific and accurate results.
Cat. No. DMAB4488S
 

 

 

One-Step Luciferase Assay System

9/08/2016 2:38:31 PM

The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: 

• Sensitive – highly sensitive detection of firefly luciferase activity. 
• Stable – the signal output is stable for more than two hours, providing flexibility with regard to incubation time 
• Convenient – simple one-step, homogeneous protocol. 
• High-throughput – one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications 
• Compatibility – works well with a variety of common media containing 0-10% serum and phenol red. 
• Instrumentation – does not require a luminometer with injectors.
luciferase reaction

New Zika Virus Antibodies & Proteins

29/07/2016 4:36:11 PM

fitzgeraldzika

 

Monoclonal Zika NS1 & Envelope Antibodies
Polyclonal Zika NS1 Antibodies
Recombinant Zika NS1 & Envelope Proteins
***Matched Protein-Antibody Pairs***
 

 

Click Here to view brochure

As formalin fixation protects the morphology of cells and subcellular structures (including nucleus shape, cellular and nuclear membranes, intercellular contacts),  it is rightfully very popular in preserving tissue samples for subsequent  immuno -staining.
 
However, cell proteins undergo not only cross-linking, but also quite some chemical modifications of amino acids upon formalin fixation, and the key is to recover the antigen (epitope) back before mmunostainingi onroutinely collected and stored tissues. 

 

There are some buffers (High, Low, EDTA, Tris, Citrate etc.  ) that allow the epitope recovery, and for about 80% of antibodies that work in blot, one can find an appropriate buffer between them. 

Unfortunately, there is one issue many people are not really aware of: using a wrong recovery buffer may still result in what looks like specific staining, and may be taken as such, but in reality is a non-specific one.
 
Therefore, the safest way to recover epitopes is to maximally reverse the fixation and chemical modifications of amino acids; and to allow the protein molecules to re-fold back maximally close to their native state. We have created, probably the most reliable epitope recovery unit, the 2100 Retriever, and thus saw the creation of a universal buffer for tissue processing as our primary task. We have accomplished it successfully with  R-Universal buffer: it properly recovers all epitopes recoverable in Tris, EDTA, High, Low, Citrate and similar buffers.  Besides standard IHC, it allows to perform multi-colour immunofluorescent staining of formalin fixed tissues and cells. Just process FFPE sections and stain them as cryostat ones!

flashBAC special offer

24/05/2016 3:24:33 PM

Get on-board with flashBAC™
Buy a pOET transfer vector and getting a free-of-charge 3 reaction flashBAC™ kit of your choice. This is the easy way to get your hands on the most advanced and superior baculovirus expression system whilst saving a bit (actually a lot) of money! 


Read More

Hot Products! CD226/TIGIT Pathway

19/05/2016 12:09:42 PM

 

Previous product spotlights have highlighted the PD-1 pathway; however, there are many other immune checkpoint pathways involved in T cell activation and anti-tumor responses. Researchers are investigating several of these targets as combinational therapies along with PD-1 inhibitors. One pathway that has been of particular interest is the TIGIT/CD226 pathway, because it acts by a novel mechanism to regulate CD8+ T cell functions within the tumor microenvironment.

The TIGIT/CD226 pathway parallels the CD28/CTLA-4 pathway that we discussed in previous newsletters. Similar to CD28 and CTLA-4, CD226 and TIGIT share ligands, CD155 (also known as the poliovirus receptor, PVR) and CD112 (Nectin-2). CD226 and TIGIT (shown here as VSTM3) can compete for ligand binding on target cells. However, CD226 and TIGIT binding have opposite results: while engagement of CD226 with CD155 or CD112 enhances T cell activation, the interaction between TIGIT and CD155 or CD112 inhibits T cell responses.

CD226

Both CD155 and CD112 are found on dendritic cells (DC) and macrophages, and both are highly overexpressed on multiple cancer cell lines and primary tumors. CD226 (also called DNAM-1) is expressed primarily on NK cells and CD8+ T cells. The CD226 glycoprotein acts as a co-stimulatory adhesion molecule, promoting Th1 differentiation, and triggering NK activation. Interactions between CD226 and the CD155/CD112 ligands play an important role in T cell and natural killer (NK) cell–mediated recognition and lysis of tumor cells.

On the other hand, CD155/CD112 binding to TIGIT (T-cell immunoreceptor with immunoglobulin and ITIM domains) directly suppresses the cytolytic activity of NK cells and inhibits T cell proliferation and cytokine production in CD4+ T cells. TIGIT also indirectly inhibits T cell responses by triggering CD155 in DCs, thereby preventing DC maturation and inducing production of immunosuppressive cytokines such as IL-10. Additionally, TIGIT has been shown to inhibit proinflammatory Th1 and Th17 responses. TIGIT can even physically block the dimerization of CD226, which prevents its costimulatory function.

CancerCell

Not surprisingly, researchers are actively developing therapeutic monoclonal antibodies to regulate these pathways. Neutralizing the TIGIT pathway is especially promising because it blocks the negative TIGIT inhibitory signal on effector T cells while simultaneously promoting the positive CD226 stimulatory signals for T cell proliferation and proinflammatory cytokine production [IFN-γ, IL-17, IL-9], leading to T cell activation. Antibodies that block TIGIT binding to CD155 have been shown to prevent TIGIT’s down-regulation of NK cytotoxicity , and to decrease tumor growth. Similarly, an anti-CD112 monoclonal has been show to exert an in vivo anti-tumor effect on breast and ovarian cancer cells, which suggests the potential of CD112 as a target for antibody therapy for cancer treatment.

TIGIT expression is strongly associated with expression of other co-inhibitory molecules; PD-1, Tim-3 and Lag-3. Thus many researchers are investigating combinations of blocking antibodies. Antibody blockade of the TIGIT pathway synergizes with Tim-3 blockade to maximally decrease tumor growth. Likewise, blockade of TIGIT signaling has been shown to synergize with PD-1/PD-L1 blockade to enhance antitumor CD8+ T cell responses in both humans and mice (see Cancer Cell figure above). In particular, antibody co-blockade of TIGIT and PD-L1 results in significant tumor clearance, and elicits tumor rejection in preclinical models.

In addition to cancer, the CD226/TIGIT pathway has been linked to several autoimmune and inflammatory diseases. For example, TIGIT engagement has been shown to ameliorate collagen-induced arthritis, while down-regulation of TIGIT exacerbates experimental autoimmune encephalomyelitis. CD226 binding to CD155 has been associated with autoimmune-linked disorders, including multiple sclerosis and type 1 diabetes, and treatment with a neutralizing anti-CD226 monoclonal efficiently inhibits activation and proliferation of T cells from patients with autoimmune diseases. Even better, naive T cells do not express CD226, so therapeutic strategies targeting CD226 would exclusively target proinflammatory Th1 and Th17 cells.

tigit

Overall, targeting the CD226/TIGIT pathway may provide a therapeutic approach that can modulate the pro-inflammatory and anti-inflammatory balance in a wide range of diseases.

BPS provides 4 unique kits for investigating the CD226/TIGIT pathway. These homogeneous kits allow screening for the identification of small molecule inhibitors and antibodies that block receptor-ligand interaction using AlphaScreen technology. BPS also offers the individual biotinylated and unlabeled proteins,  

Antibody Labelling

23/03/2016 2:14:54 PM

New Guide to Antibody Labelling

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Multiplexed assay for epigenetics.

18/03/2016 9:32:03 AM

qPCR is holding you back. Break free with a multiplexed assay for epigenetics.

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Quicker Ab-Oligo conjugates with Thunder-Link PLUS


We have a new kit in our Thunder-Link® range!

 

For almost 3 years, our Thunder-Link® technology has allowed researchers to quickly and effectively conjugate oligos to antibodies. We are very excited to introduce the newest kit in this range, Thunder-Link® PLUS!

We have taken on board observations from our customers to build upon the established Thunder-Link® kit, to achieve more efficient conjugations, and better results in even less time.

The most notable improvements include:

  • Activation time halved - now only 30 min      
  • Conjugation time reduced from overnight to      
    1 hour
  • Higher antibody and oligo recovery     
  • More robust clean-up procedure    
  • Antibody fragments and small proteins now compatible    

We have retained our excellent batch-to-batch reproducibility and scalability, however, there is no need for specialist conjugation equipment. 

Click on the boxes on the left to view more resources, or more info on the kits here.

 



 

 

        Press Release

 

        Product page

 

           View the protocol

 

           Ask us a Question

 

 


 

 

 

 

 

 



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Tandem Dyes

15/01/2016 12:29:29 PM

Trouble with Tandems? 

The use of tandem dyes means a larger color portfolio can be made available to researchers using a limited number of lasers in a multi-color experiment. Traditionally creating tandem-antibody conjugates can be time-consuming and complex, with both the conjugates, and the tandem labels themselves being difficult to produce.
 
Our Lightning-Link® kits allow quick and easy conjugation of labels to antibodies, and we have a selection of tandem dyes available in this range. The tandem label is already produced for you, and lyophilized to increase stability pre-conjugation, meaning antibody-tandem conjugates are possible with just 30 seconds hands on time. 

Sound good? Click here for our full article on Top Tips for using Tandems.

Breast cancer cells grown in 2D can easily be killed by low doses of chemotherapeutic drugs or low doses of radiation. If those same cells are grown in 3D, they are resistant to the same doses of chemotherapeutic drugs or radiation, just like cancer as its found in the body. In this way, cells grown in 3D are more valid targets for testing and discovering new drugs to treat cancer. Another benefit of testing drugs in three dimensional cell culture versus two dimensional cell culture is that cells in 3D form multi-layers of cells whereas cells grown in 2D form a monolayer of cells that is spread very thin on a plastic surface. When testing a drug in 2D, it needs only to diffuse a short distance across the cell membrane to reach its intended target. In 3D, the situation is more realistic and a drug needs to diffuse across multi-layers of cells to reach the cells on the inside of a microtissue.

3D Tissue Growth CollaGel Hydrogel

 

publication: http://onlinelibrary.wiley.com/doi/10.1002/adma.201503001/full 

This webinar on immunoassay development, validation and sample analysis gives a great overview of developing fast, simple and cost-effective immunoassays using the unique SPARCL proximity assay technology from Lumigen alongside highly stable HRP conjugates developed with Lightning-Link® labeling technology from Innova Biosciences.

 

                                                                                  Watch the webinar

You can also download a PDF copy of the presentation here.

3D Tissue Engineering, Collagen, In Vitro Models, Cell Lines, Staining, Fluorescent Protein Expression, Growth Medium, Antibodies, Kits and Cell Culture.

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New! Desmosine, fMLP and A1AT assays

25/11/2015 4:06:31 PM

We have teamed up with Mologic, a specialist diagnostic development company, to offer a range of high-quality assay kits.


The B.I.T.S.TM ELISA and lateral flow kits are easy to use and provide fast, accurate, and high quality assays, which require no sample preparation.


They contain everything required for ELISA and lateral flow assays, including high quality, stable enzyme or gold conjugates, produced using the world-leading Lightning-Link® conjugation technologies from Innova Biosciences.


The first products launched in the B.I.T.S.TM assay range are the Desmosine ELISA Kit, fMLP ELISA Kit, and the Human A1AT Lateral Flow Kit, with more kits due for release throughout 2015/2016.


For more information on the different kits available, please click on the products listed below:

Desmosine ELISA Kit

fMLP ELISA Kit

Human A1AT Lateral Flow Kit

To order these kits please contact us to request a quote stating the type of kit and quantity you require.

 

In the meantime if you would like more information about these kits or have any questions please get in touch with our technical support team  at Innova attechnical.enquiries@innovabiosciences.com.

Find out more about ELISA Assays and Lateral Flow in our application guides below:

An Introduction to ELISA

Lateral Flow Immunoassays

BITS ELISA

Viewing methylation steps in time

24/11/2015 12:24:28 PM

Monitoring DNA methylation at the single-cell level

 

In this week’s Cell publication, researchers from MIT and Whitehead Institute for Biomedical Research document the introduction of a green fluorescence protein-based reporter method to study DNA methylation patterns in individual cells.  This technique captures the endogenous methylation state of promoters and non-coding regions, and can trace methylation changes both in vitro and in vivo.

dnamethylation

Keeping track on your reporters

Browse our range of state-of-the-art reporter products to assist you with looking for changes in expression. 

Choose from:  BiotinGreen fluorescence proteinRed fluorescent proteinLuciferase and  Beta-galactosidase,

 

 

 

 

 

Quantify GFP or RFP by Simple ELISA          

Now you don't need a flow cytometer to quantify GFP or RFP. Our easy-to-use ELISA Kits allow you to quantify GFP or RFP right on your benchtop using a standard microplate reader.

  • The GFP ELISA Kit is designed to accurately measure enhanced variants of Aequora victoria GFP, including EGFP, ECFP, EBFP and EYFP.  
  • The RFP ELISA Kit quantifies a variety of red fluorescent protein variants including TagRFP, TurboRFP, DsRed, mCherry, mKate, mOrange, mPlum, mRuby, mStrawberry, and tdTomato 

 

cblgfp

 

 

TECH CORNER: Storing GFP Lysate for Later Testing   

Q: After we have prepared our GFP lysate sample, can we freeze the lysate for later testing?

 

A: Click here for answer 


For more Frequently Asked Questions on Cell Signaling click here.


Don't see your question answered on our FAQ page? Contact our scientists here

Full Moon Free Ab Array

18/11/2015 4:48:54 PM

We are currently running a special promotion – Get one free set of antibody arrays (Chromatin or p53) with the purchase of an Antibody Array Assay Kit.  More details can be found on here

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  1. 1. Reproducible results
    ProcartaPlex® assays are based on the principles of sandwich ELISA: two highly specific antibodies recognize different epitopes on the same protein. With antibody assays, reproducible results come from reliable manufacturing. Affymetrix operates a world-class hybridoma facility where ProcartaPlex antibodies are manufactured, enabling the delivery of consistent, reproducible, and high-quality assays.
  2. 2. Correlation to ELISA
    Switch easily from ProcartaPlex assays to highly validated ELISA, and vice-versa, with reliable results. A majority of ProcartaPlex assays use the same antibody pairs as our Platinum ELISA, resulting in high correlation (R2 > 0.9) between these two assay platforms.
  3. 3. Scalability
    Obtain the same results regardless of which ProcartaPlex product format used. Scale down easily from a high- to a low-plex panel or to a single-plex assay.
  4. 4. Flexibility
    Get exactly the panel you want. More than 90% of ProcartaPlex assays are combinable with one another, allowing for the creation of highly individual and high-plex panels. Affymetrix currently offers the largest published panel with a 63-plex.1,2,3,4
  5. 5. Individually custom-built
    Don't accept restrictions to your panel design based on bead conflicts. We change overlapping bead regions in your custom Mix & Match panel.

See a list of available analytes  

Design your own panel

Find out more

Or e-mail us at info@huntingtree.co.nz with enquiries.  

Spotlight on Stem Cells

15/10/2015 3:26:43 PM

Biorbyt offer a specialist range of antibodies for stem cell research 

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 Reactive oxygen species (ROS) are continually produced during metabolic processes, and ROS generation is normally counterbalanced by the action of antioxidant enzymes and other redox molecules. However, excess reactive oxygen species must be promptly eliminated from the cell by a variety of antioxidant defense mechanisms. Excess ROS can lead to cellular injury in the form of damaged DNA, lipids and proteins.

Here we discuss how to measure ROS in a variety of samples types. Continue reading for more details. 

 

Read More

Green Fluorescent Protein(GFP) is a 27kD protein derivedfrom the Aequorea victoria (A.victoria) jellyfish. It is a naturallyfluorescent protein which hasrevolutionised life science andhas been used to study proteinsin a wide range of applications.GFP has been used extensivelyto monitor gene expression; tolocalise proteins at the cellularand sub-cellular level; to trackmetastatic cells and as a tag toidentify proteins in vitro.

Read More

Detection of ROS directly can be difficult, since the half-life of most free radicals is so short. A more reliable and well-published method is to detect the resulting damage caused by ROS. Proteins are a common target of ROS and provide an easy, sensitive method for testing oxidative stress levels.

In this edition we discuss various ways to test for specific protein damage markers. Continue reading for more details.



Read More

BPS Bioscience: Immunotherapy

3/06/2015 11:59:53 AM

Immunotherapy is the treatment of disease by inducing, enhancing or suppressing an immune response. T-cell activation and inactivation requires the coordination of a multitude of co-inhibitory and co-stimulatory signals and most immunotherapies modulate hese signals.

Read More

Veterinary Immunoglobulins

4/05/2015 2:42:41 PM

Nordic logo

Nordic-MUbio has developed an ever-expanding range of high quality immunological research tools, manufactured to strict ISO 9001 guidelines, to provide both reassurance and the best possible tools for your research.

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Introducing CypExpress

17/03/2015 3:09:13 PM

CypExpress™ is a stable dry biocatalytic system containing both an individual recombinant human P450 and recombinant oxidoreductase along with cofactors and antioxidant systems produced in a eukaryotic expression system.

Read More

New Video Protocols

8/03/2015 9:36:20 PM

Our range of technologies allows you to label your biomolecule in just a few simple steps with very little hands-on time.

To help you quickly understand our conjugation protocols (and to see how easy they really are!), we have developed new demonstration videos and redesigned our labeling kit protocols to help you achieve optimum conjugates for your experiment.



Read More

The simultaneous staining of two antigens can be a very powerful technique to study antigen expression but is traditionally time consuming and highly complex. This is due to a requirement for different primary antibody species and highly specific secondaries to eliminate cross-reactivity.



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Oxford Expression Technologies Ltd has pleasure in announcing the market launch of baculoFECTIN 11;

 

baculoFECTIN II Transfection Reagent

Excellent Transfection Efficiency – Easily Produce High Titre Virus Stocks

 

baculoFECTIN II is a transfection reagent ideally suited for use with insect cell lines.

Although baculoFECTIN II is optimised for use with the flashBAC system in Sf9 insect cells it is compatible with other baculovirus expression platforms and cell lines.

 

High Efficiency – Effective transfections with reliable reproducibility

High Titre Stocks – Easily produce high titre virus stocks (1 x 108 pfu/ml) ideal for further work and protein expression.

 

When working with insect cells and the baculovirus expression system the co transfection step is a crucial step which can often cause problems even for the most experienced researcher.

Working with Oxford Expression Technologies you have access to over 20 years of experience with the baculovirus system.

 

Please contact us if you would like to discuss your co transfection projects or if you have any questions regarding baculoFECTIN II, we would be happy to help.

Detection of ROS directly can be difficult, since the half-life of most free radicals is so short. A more reliable and well-published method is to detect the resulting damage caused by ROS. Proteins are a common target of ROS and provide an easy, sensitive method for testing oxidative stress levels.

In this edition we discuss various ways to test for specific protein damage markers. Continue reading for more details



Read More

Membrane Antibody Arrays

11/12/2014 11:42:39 AM

Are you tired of performing multiple Western Blots? Try ourC-Series Membrane Antibody Arrays and you can detect 10 to 274 proteins on one membrane without running gels or performing transfer.
Stop Stripping & Re-Probing Westerns!
C-Series Array Features
  • Simultaneous detection of multiple proteins
  • High specificity (uses sandwich ELISA antibody pairs)
  • More sensitive than Western Blot (pg/ml levels)
  • No special equipment needed
  • Compatible with any chemiluminescent imaging system, including Li-Cor Odyssey & Typhoon.
  • Easy to use, no training required
  • Results obtained in 1 day

New kits enable customers to conjugate primary antibodies in 20 minutes

Read More

Newest Assays for Oxidative Stress

6/11/2014 2:28:36 PM

OXofr Biomedical Research introduce several new assay kits for this important research area.

Read More

Innova Biosciences Ltd will be holding its next webinar on 29th October 2014. In this webinar, Alastair Carrington, Corporate Business Specialist at Innova, will discuss how to overcome common issues associated with colorimetric drug screening assays for measuring phosphate-generating enzyme activity. The unique properties and applications of Innova Biosciences' proprietary PiColorlock phosphate detection reagent will also be discussed.

 Please find more information here: http://bit.ly/1Cuq7iV

Read More

Many Biorbyt products have related products against the same target, making it convenient for you to choose reagents for another technique. Just click the ‘Related Products’ tab above the product.

For example, our Human IDO1 gene (orb92761) has the following associated tools:

Dissolving peptides

 

shRNA

17/10/2014 11:19:44 AM

Silence Your Gene, Promote Your Research, Guaranteed knockdown!

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Protein Quantitation Assay

Our new High Sensitivity Protein Quantitation Assay Kit utilizes a fluorogenic detection dye to measure primary aliphatic amines (peptides or proteins) in various samples with much greater sensitivity than the conventional colorimetric protein determination methods. 

 

More information 

Protein Phosphorylation Reagents

Phosphoproteins represent an important part of cell signaling research. Standard methods used in protein analysis are often not sufficient for use with phosphoproteins. We have developed reagents that are specifically designed to improve your phosphoprotein research:

  • PhosphoBLOCKER Blocking Reagent was designed to provide superior blocking compred to dry milk or serum; it enhances low level phosphoprotein signals without increasing background, and it preserves phosphoproteins during Western blotting.
  • Our Phosphoprotein Purification Kit provides enrichment of phosphoproteins by affinity purification using a single-step matrix.
  • Our Phospho Antibody Stripping Solution removes phosphoantibodies from blots without affecting the immobilized proteins, allowing you to re-probe with the same or a different antibody.   

 

His-Tag Protein ELISA Kit

Our His-Tag Protein ELISA Kit allows you to detect and quantify His-tagged protein samples simply and reliably by comparing your unknown samples to a known recombinant standard.

Sensitivity range of the kit is 4 µg/mL to 1 ng/mL, or 950 nM to 50 pM of 6xHis-tag residues.

More information 

 

 ELISA Plate

 

Protein Isolation and Extraction Reagents

We have developed a number of reagents and kits to facilitate the extraction and isolation of proteins from cells:

How To Measure Antioxidants

19/09/2014 12:01:34 PM

Enhanced TBARS Method from Oxford Biomedical Research Inc

  • Works with all anti-oxidants
  • Works with hydrophobic samples
  • Works with hydrophilic samples
  • Linear sample dilutions
  • Chromogen absorbs in visible region
Read More

Free Webinar!!!  Even if you can't make the time (!!!) register and you will be sent a video .

Gold nanoparticles: optimization of conjugates and the importance of size, shape and surface properties in different applications

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innovalogo

Some of our fluorescent labels, which previously were available in the standard Lightning-Link format, have now been made available in the Lightning-Link Rapid form. 

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Scientist Holidays

6/08/2014 3:28:37 PM

graphicholidays

BPS is excited to announce two unique assay kits for screening inhibitors of histone demethylases FBXL10 (KDM2B) and FBXL11 (KDM2A). FLBL10 and 11 play critical roles in embryonic development and cellular differentia-tion, and both are potential targets for cancer therapy.  These two enzymes preferentially recognize the dimethyl form of lysine 36 on Histone H3 (H3K36me2). Although methylation of histones is usually associated with transcriptional repression, methylation of the H3K36 site by SETD2 (BPS #53019) is associated with gene expression.  Modulation of FBXL demethylase activity has been shown to regulate cell proliferation and senescence. These kits are exclusive to BPS!

 

Read More

Hot Products #1:    Immunotherapy products! 

BPS announces even more products for our new immune-therapy line, including proteins for the CD47:SIRPα, ICOS:B7-H2, and B7.1/B7.2:CTLA4 pathways. We’re especially enthusiastic about the two neutralizing antibodies for either CTLA4 or PD-L1. These antibodies can be used as inhibitors to block CTLA4 binding to either B7.1 or B7.2, or to block PD-L1 binding to PD-1, respectively.

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Our popular PCSK9 assay kit is now available in a convenient, homogeneous TR-FRET format, which makes it more attractive to customers performing high throughput screening.  We also now offer the mutant (D374T) PCSK9 protein and assay kit---this mutation causes increased affinity of PCSK9 for the LDLR substrate. These kits are exclusive to BPS! 

Read More

OET blog

5/08/2014 4:10:08 PM

I have pleasure in providing a link to a URL for a new Blog from Oxford Expression Technologies Limited. (This is also accessible from the Home Page of our website (www.oetltd.com [1]).)

 http://oetltd.wordpress.com/ [2]

 

We hope you  find this Blog of interest.

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Gold nanoparticles are used in a variety of applications. In addition to lateral flow point-of-care assays, particles are also used in microscopy, flow cytometry, Raman spectroscopy and dynamic light scattering.

In all of these applications the use of high quality particles is vital. There are many sources of gold, both commercial and DIY, however InnovaGOLD has been rigorously tested against market-leading brands and shown to be superior.

  • Most uniform shape
  • Low co-efficient of variation in terms of size
  • High efficiency antibody binding for maximum sensitivity
  • Available at high concentrations to simplify assay development
Read More

Detect many forms of DNA damage

11/07/2014 3:05:09 PM

DNA damage can take many forms and has been implicated in age-related development of a variety of cancers including colon, breast and prostate. In this edition we discuss various assay kits to help measure DNA damage in a quick and reliable way. Read on for further details

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Labor Day

1/05/2014 10:47:41 AM

Happy Laboratory Day to all Lab Scientists -from Juha Wahlstedt  on  http://clinical-laboratory.blogspot.fi/ 

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Antioxidants are important in the study of various disease states, nutrition, and general well-being. 

 We have recently expanded our selection of tools to help facilitate your antioxidant research, whether it is measuring endogenous antioxidants, the antioxidant capacity of certain biomolecules, or the efficacy of potential antioxidant compounds. 

Read More

Immuno-PCR

1/04/2014 10:38:53 AM

Immuno-PCR
Combining ELISA and PCR techniques for enhanced assay sensitivity


Read More

The ongoing discovery of disease associated biomarkers is driving the demand for reliable antibodies to facilitate research in the biomedical field. Molecular biomarkers help to accelerate our understanding of the cellular and molecular mechanisms of diseases in terms of initiation, development and progression. In addition, specific biomarkers may have an important role in disease diagnosis, monitoring, prognosis and therapy.

Read More

IUBMB conference

25/03/2014 12:10:50 PM

Dear Friends and Colleagues,

The first time IUBMB annual conference comes to Taiwan. On behalf of the Organizing Committee, we would like to extend to you a cordial invitation to participate in the 15th IUBMB-24th FAOBMB-TSBMB International Conference, being held from October 21-26, 2014 at Academia Sinica, Taipei, Taiwan.

The 2014 conference, jointly organized by International Union of Biochemistry and Molecular Biology (IUBMB), Federation of Asian and Oceanian Biochemists and Molecular Biologists (FAOBMB), Taiwan Society of Biochemistry and Molecular Biology (TSBMB) and Academia Sinica, has the theme of “Biochemistry and Molecular Biology in Transition: from Basic to Translational”. For several decades, many breakthrough discoveries in biochemistry and molecular biology have contributed comprehensively to the advances of understanding the fundamental biological processes, which, in turn, have set the pathway for translating basic scientific discovery into application. In line with this theme, all scientific sessions will focus on emerging research areas or technologies in basic scientific discovery, as well as new synergies in translational sciences. The program has been selected not only to capture a snapshot of the state-of-art findings in these challenging areas but also to fuel the translation of life sciences.

In addition to the excitement of breakthrough discoveries, the conference also provides a forum for sharing your insightful research, as well as a great opportunity to network with your fellow professionals and establish interdisciplinary partnerships. Please join us for what will be a wonderful experience on the cutting-edge sciences.

 

For exhibitors, i

f you are interested in demonstrating your company

/

institutions, products and/or services to decision makers and influences in the relative fields, we are confident that attending the 15th IUBMB Conference will meet your target. We are now providing a special discount, please contact us to learn more about the exhibits. 

 

For more information, please visit us at http://bmb2014.sinica.edu.tw/.


We look forward to welcoming you to Taiwan, Formosa – the beautiful island, for an inspiring and vibrant conference you will never forget.


Sincerely,

Andrew H.-J. Wang, Ph.D.
Chairman, Organizing Committee

 

Ming-Daw Tsai, Ph.D.
Co-Chairman, Organizing Committee

 

 

--

Shan-Chi Ku (古珊綺), Ph.D.
Manager
Office of International Collaboration
National Research Program for Biopharmaceuticals (NRPB)

Institute of Biological Chemistry
Academia Sinica, TAIWAN
Tel: +886-2-2787-5270 ext.22
Fax: +886-2-2788-9759

 

Cells constantly undergo damage and stress from external and internal factors. Our Cell Health Assays provide a simple, user-friendly way to measure various parameters of live, dead, or dying cells, as well as cells under stress. 

Read More

Phosphate Detection System

18/03/2014 11:24:41 AM

PiColorLock™ Gold is a non-radioactive, superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes

Read More

Oxford Biomedical Research (OBR) has recently developed a room temperature TBARS method that eliminates the requirement for a high concentration of a strong acid and cuts reaction time in half.  By running the assay at room temperature, low levels of interfering substances are generated. This results in more accurate, precise and reliable TBARS measurements.

Read More

Optimal protein expression requires accurate virus titres. The baculoQUANT all-in-one titration kit from Oxford Expression Technologies enables you to quickly determine your virus titres.

Read More

Make your own Tissues Arrays

11/09/2013 4:52:55 PM

Make Tissue Arrays in your own lab. 

When you need them and how you need them.

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Oxford Biomedical Research  continues to develop new user-friendly ELISA Kits and Enzymatic Activity Assays to study various lipoprotein metabolism pathways. Here we highlight the newest research assays to reliably study a variety of these proteins. 

Please note: These assays are for research use only; they are not for clinical or diagnostic use



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Problems with standard gold labeling procedures•

Naked gold particles extremely difficult to work with – crash out of solution if incompatible buffers used or wrong pH.

 Technically difficult to optimise a gold conjugate requiring many trial conditions – for instance altering pH, antibodyconcentration + buffer formulations.

Standard labeling involves non-covalent bonds which leads to conjugate stability issues.

InnovaCoat GOLD – overcoming the problems of standard gold labeling procedures

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Oxford Biomedical Research are constantly developing new innovative assays to improve the reliability of oxidative stress testing. We have recently developed new Competitive ELISA kits that provide more sensitive and reliable detection, especially when testing plasma or serum. These assays are also more user-friendly for cell and tissue lysates.  

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Antibody Microarray

29/07/2013 3:08:37 PM

Antibody Array provides a high-throughput platform for efficient profiling of protein expressions.  Our comprehensive Phospho Explorer Array and Explorer Array allow investigators to examine hundreds of proteins in a single experiment, while the Pathway Antibody Arrays and Phosphorylation Antibody Arrays are designed for researchers to study highly relevant proteins in their specific research fields. 

The antibodies are covalently immobilized on high quality glass surface coated with our proprietary 3-D polymer materials to ensure high binding efficiency and specificity.  Each array includes well-characterized antibodies, positive controls and negative controls. To maximize data reliability, eachantibody is printed with six replicates.  The arrays utilize fluorescent detection and can be scanned on all microarray scanners that are compatible with 76 x 25 x 1 mm (3 in. x 1 in. x 1mm) slides. 



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We continue to develop new assays to facilitate the important research area of metabolism research. Read on for details on the newest assay kits to join our growing portfolio.

Please note: Our assays are not for clinical or diagnostic use.



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Activation Assays

21/05/2013 11:54:50 AM

Small G-protein activation assays measure the GTP-bound form of the protein from a cell or tissue extract.  Cytoskeleton offers activation assays for Rho and Ras GTPase family members in two formats: the traditional pull-down assay and a G-LISA assay.

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Renal Function Research Made Simple

10/05/2013 11:51:29 AM

 Our kidneys have an important function: to remove wastes and water from the blood to form urine. Kidney failure can result from many factors including high blood pressure, diabetic kidney disease, and the overuse of medications.

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Tricky Transfections

10/05/2013 11:25:32 AM

Tricky transfections can be a real nuisance. Oxford Expression Technologies Ltd provides a wide range of products and services to help.

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From the 1st May until 30th June we will be offering 20% off the list price of our flashBAC kits. 

This includes:

  • flashBAC 3,5,24 reaction kits.
  • flashBAC GOLD 3,5,24 reaction kits.
  • flashBAC ULTRA 3,5,24 reaction kits
  • flashBAC PRIME 3,5,24 reaction kits.
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Evolution

4/04/2013 12:29:27 PM

Clearly a quiet week in the office and i have time to look around.  Really enjoyed this site - http://wtfevolution.tumblr.com/

Hope you do too! 

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Olaf Pharmaceuticals develops high quality extracellular matrix products for various purposes including cell attachment, cell growth, cell differentiation, cell migration, Stem cell research, tissue engineering and tissue morphogenesis. Olaf Pharmaceuticals is currently offering a range of Type I collagen products derived from various sources.  We also develop tissue-engineered products for a number of research, drug delivery -related applications, such as vasculature.

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Great Sites

27/03/2013 11:15:41 AM

A quick link this week to a website that we found while looking around which has great science memes. If you're feeling brave, go to the sister-site which has really good articles but a less socially acceptable name. All part of making science more accessible.  See it here 

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SuperBiochips Array

20/03/2013 12:19:55 PM

Tissue Array (or Tissue MicroArray) is a method of relocating multiple tissues from conventional histologic paraffin blocks so that tissues from multiple patients can be seen on a same slide.
We are pleased to be able to offer the range of tissue array products from SuperBioChips.  Please contact us for more details or visit the website here


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B-Cell Activation

20/03/2013 11:48:15 AM

Activating B cells with Anti-IgD eliminates problematic interference from high levels of circulating serum IgM experienced with Anti-IgM B cell activation.

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  • End user chooses oligo sequence and antibody.
  • Oligo sizes (HPLC purified) between 10-120 bases.
  • Amine group positioned at the 5’ or 3’.
  • Positive control included.
  • All necessary components for a successful conjugation supplied.
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In a perfect world, everything would go smoothly, we’d only work with clean samples and they could be harvested, stained and acquired all in a day’s work. Unfortunately, science is never predictable.

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Antioxidants are important in the study of various disease states, nutrition, and general well-being.  

We have recently expanded our selection of tools to help facilitate your antioxidant research, whether it is measuring endogenous antioxidants, the antioxidant capacity of certain biomolecules, or the efficacy of potential antioxidant compounds.

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Reliable Assays for DNA damage

15/11/2012 10:18:43 AM

DNA damage can take many forms including oxidative damage, hydrolytic damage (loss of bases) and strand breaks. Continuous DNA damage has been implicated in age-related development of a variety of cancers including colon, breast and prostate.

In this article we discuss various assay kits to help measure DNA damage in a quick and reliable way. Read on for further details.



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Interactive Biological Pathways

7/11/2012 11:43:28 AM

Want to know more about the biological pathways that intrigue you?  Check out the pathways now live at eBioscience.com.

  • View illustrative and colorful pathway diagrams
  • Learn about the biology behind each pathway
  • Access further reading with recent publication references
  • In pathway links to available products for the proteins associated
    with each pathway 

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New ELISA kits available

1/11/2012 10:47:39 AM

eBioscience have announced the launch of five new ELISA assays.

  1. Human HSP27 (Heat Shock Protein -27) Platinum; launched BMS2086
  2. Human C1q (Complement Factor 1q) Platinum; launched BMS2099
  3. Human C3a (Complement Factor 3a) Platinum; launched BMS2089
  4. Mouse Ig Isotyping Instant, 2 precoated Plates; 88-50660-22 
    1. (IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM, light chain kappa and lambda);  Launch early Nov.
  5. Mouse TNF-alpha High Sensitivity Kit; Launch End of November; BMS607HS 
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Choosing a Buffer for Flow Cytometry

16/10/2012 10:09:15 AM

Choosing the correct buffer for flow cytometry?

Buffers, buffers and more buffers. How do you know which buffer is the right one to use with your staining protocol? Easy, it all depends. The first question you must ask is where are the markers of interest located, surface expressed or intracellular?

Generalized protocol comparison for flow cytometry

1) Surface staining only,

2) surface + cytoplasmic (which include secreted proteins), and

3) surface + cytoplasmic (which include secreted proteins) + nuclear.

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Label as little as 10μg of antibody or protein in minutes!
Having problems getting that conjugated antibody?
Innova Biosciences can help you find the missing link!



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Tips & Tricks

New Product Launch – OneComp eBeads

Why is compensation important?

 

Compensation is necessary when performing flow cytometric analysis due to the overlap of emission spectra from fluorochrome conjugated antibodies.

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Oxford Biomedical Research, new kit for Oxidative Stress - Catalogue No EA85

Isoprostanes (IsoPs) are radical-mediated byproducts of lipid peroxidation and are widely regarde as the "gold standard" for assessment of oxidative stress in vivo, and multiple studies have shown that normalized values for urinary Isoprostanes correlate very well with plasma values. 



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Cell Biolabs Inc continue to develop new assays to facilitate the important research area of lipoprotein metabolism. Read on for details on the newest assay kits.

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Annexin V Reagents and Kits

28/08/2012 1:54:12 PM

Annexin V is a membrane protein which binds directly to phosphatidyl serine (PS) which is normally expressed internally, but upon apoptosis initiation moves to the cell surface.

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Oxford Biomedical Research (OBR) has recently developed a room temperature TBARS method that eliminates the requirement for a high concentration of a strong acid and cuts reaction time in half. 

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Ligation Independent Cloning

3/08/2012 11:30:33 AM

High throughput construct preparation & gene expression
now 
Faster and Simpler

OET launches the enhanced Ligation Independent Cloning (LIC) versions of the transfer plasmids to go with their flashBAC baculovirus expression vector range


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Welcome

17/07/2012 4:39:16 PM

Welcome to the updated Huntingtree Bioscience Supplies website.  We have made some major changes to help you find the reagents you need.  Just enter your key words in the search box and you can search our product ranges of over 70,000 items.  We would appreciate any comments or tips you have and look forward to hearing from you.  

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The evolution of phospho ELISAs leaps forward with the efficient sensitivity and flexibilityof InstantOne ELISAs

.• Fast: Less than 1/3 the time of traditional ELISAs

• Easy: 1/4 the effort with only 1 wash step

• Flexible: Enables the detection of total and/or phospho protein levels of a singleprotein up or down a pathway on a single plate

• Sensitive: Meets or exceeds industry standards with only 2-3,000 cells needed per well

• Compatible: Colorimetric detection; utilizes standard plate readers

• Complete: Over 50 kits available

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Protein Isolation and Extraction

10/07/2012 3:58:57 PM

NEW! Protein Isolation and Extraction Reagents from Cell Biolabs

We have recently developed a number of reagents and kits to facilitate the extraction and isolation of proteins from cells:

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Secondary antibody amplification hypothesis questioned

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Total Antioxidant Power Assay

22/05/2012 3:06:07 PM

The well-established Total Antioxidant Power Assay from Oxford Biomedical Research has just been made even better by reducing the cost per sample, providing a broad dynamic range and expanding the range of samples.  

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