Methods for measuring inorganic phosphate (Pi) using dyes such as Malachite Green are well known, however the dye-Pi complexes are prone to precipitation and the reagents are very acidic which can lead to non-enzymatic hydrolysis of many phosphorylated substrates.

At the heart of the Innova Biosciences' range of phosphate detection products is PiColorLockTM a unique phosphate detection reagent that overcomes these common issues. The formulation of PiColorLockTM affords enhanced assay linearity, dynamic range and color stability, and it is also possible to work with unstable substrates that give high non-enzymatic background signals with other acidic dye-based detection reagents.

PiColorLockTM can be purchased singularly or as part of our ATPase and GTPase assay kits.

For more information about our range of phosphate detection products, take a look at our video below: 

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The PiColorLockTM reagent is often used with unstable substrates (e.g. ATP, GTP) that give rise to non-enzymatic background drift with time. The unique stabilizer, provided in the PiColorLockTM phosphate detection system, blocks this non-enzymatic breakdown generating a stable low background. The stable signal can therefore be read up to several hours after the reaction has ended.