Direct conjugation for dual staining in immunohistochemistry
Direct conjugation for dual staining in immunohistochemistry
13/02/2015 9:01:32 PM
Direct conjugation for dual staining in immunohistochemistry
The simultaneous staining of two antigens can be a very powerful technique to study antigen expression but is traditionally time consuming and highly complex. This is due to a requirement for different primary antibody species and highly specific secondaries to eliminate cross-reactivity.
Lightning-Link® antibody labeling kits allow easy conjugation of primary antibodies with only 30 seconds hands-on time, eliminating the need for secondary antibodies and avoiding cross-species reactivity.
The IHC data below shows that it is possible to obtain very good staining whilst avoiding the use of secondary antibodies, therefore saving time and reagent costs.
Lightning-Link® kits were used to conjugate mouse anti-human CD20 to HRP and rat anti human CD3 to Alkaline Phosphatase. Both antibodies were then used simultaneously to stain formalin fixed paraffin embedded sections of human lymph node. CD20 positive cells are visualised with DAB and are stained brown, and CD3 visualised with Fast Red and stained Red.
It is with great pride and immense pleasure that we introduce the Creative Bioarray QualiStem® Alkaline Phosphatase Live Staining Kit which provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell to be used for follow-up studies
Since the Monkeypox was first detected on 7 May 2022 in the United Kingdom, more and more cases have been confirmed in non-endemic countries, Creative Biogene decided to do our share for supporting the scientific community against this disease and would like to introduce our Monkeypox Virus Real Time PCR Kit.