High throughput construct preparation & gene expression now Faster and Simpler
OET launches the enhanced Ligation Independent Cloning (LIC) versions of the transfer plasmids to go with their flashBAC baculovirus expression vector range
Ligation Independent Cloning (LIC)
• No DNA ligases- saves time • Directional cloning of target gene • Rapid cloning of multiple inserts into multiple vectors • High cloning efficiencies
• Facilitates quick and simple gene transfer into flashBAC system • Driven by polh or p6.9 promoters • His tag for efficient protein purification • gp64 secretory signal to enhance protein secretion • TEV cleavage site for removal of fusion peptide
