ace2assaykits

 

The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein receptor binding domain of SARS-CoV-2 and ACE2 receptor may offer some relief against this novel viral infection.

BPS offers two new kits to screen for inhibitors binding to the Spike protein (#79331) or the ACE2 receptor (#79936). These sensitive kits can detect inhibition of Spike:ACE2 binding in a 96-well format using chemiluminescent detection. First, ACE2 or SARS-CoV-2 Spike-Fc are attached to 96-well plates through nickel-His tag or anti-Fc:Fc tag interactions. Next, corresponding ligands are incubated with the coated plates. Finally, the plates are treated with HRP-labeled antibodies followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader. BPS has developed these kits with either the Spike or ACE2 protein fixed to the plate, to allow greater flexibility in your experimental design.

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SARS-CoV-2 Spike binding to immobilized ACE2 using the ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit (BPS Bioscience #79936). Luminescence was measured using a Bio-Tek fluorescent microplate reader.

ACE2:SARS-CoV-2 Spike Inhibitor Screening Assay Kit

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ACE2 binding to immobilized SARS-CoV-2 Spike using the SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit (BPS Bioscience #79931). Luminescence was measured using a Bio-Tek fluorescent microplate reader.

SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit

Angiotensin converting enzyme 2 (ACE2) is an exopeptidase that catalyzes the conversion of angiotensin II to angiotensin 1-7 and L-phenylalanine. Angiotensin II is part of the classical renin angiotensin system (RAS), a hormone system that regulates fluid balance, blood pressure and maintains vascular tone. ACE2 has been also proved to be the receptor for the human respiratory coronavirus NL63, the SARS-coronavirus (SARS-CoV) and the novel coronavirus 2019-nCoV/SARS-CoV-2.

The ACE2 Inhibitor Screening Assay Kit is designed to measure the inhibitory activity of compounds against ACE2 for screening and profiling applications.