Cells constantly undergo damage and stress from external and internal factors. Our Cell Health Assays provide a simple, user-friendly way to measure various parameters of live, dead, or dying cells, as well as cells under stress. 

NEW! Hypoxia Assays

Cell hypoxia, or low oxygen condition, is a normal

physiological response to certain body stressors, but it also can be a symptom of pathological conditions and is often used as a marker for tumor cells. A primary hypoxia marker is Hypoxia-inducible factor-1 (HIF-1), an oxygen-sensitive transcription factor.  

• Our HIF-1 Alpha Sandwich ELISA measures HIF-1 in any protein sample including tissue homogenates, whole cell lysates, and nuclear extracts.
• Our HIF-1 Alpha Cell Based ELISA provides rapid detection of HIF-1 Alpha levels in intact human, mouse or rat cells.
• Our HIF-1 Alpha DNA Binding Activity Assay detects activated HIF-1 in complexes from human, mouse or rat protein samples.

Cell Proliferation Assays

Cell proliferation is essential for a variety of applications such as optimizing cell culture conditions and determining activity rates of cytokines and growth factors. We offer a variety of user friendly methods to measure cell proliferation.

• MTT Cell Proliferation Assay is a well-published colorimetric method.
• WST-1 Cell Proliferation Assay Reagent provides colorimetric detection in a simpler single-step format. 
• Fluorometric Cell Proliferation Assay Reagent measures proliferation with even greater sensitivity than colorimetric detection. 
• BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation, using an anti-BrdU antibody.
• PCNA ELISA Kit detects PCNA from mouse, rat and human samples. 

Cellular Senescence Assays

Cells that are damaged or old can become senescent, which causes changes to molecular and cellular structure, and possibly death. When these senescent cells change for the worse they can possibly induce nearby cells to become senescent.

• 96-Well Cellular Senescence Activity Assay measures activity of senescence-associated 

ß-galactosidase in a convenient fluorescence plate-based format. 

• Cellular Senescence Staining Kit stains cells for senescence-associated ß-galactosidase.
• Cellular Senescence Flow Cytometry Assay uses a fluorogenic substrate to measure senescence-associated ß-galactosidase activity via flow cytometry or fluorescence microscope.

Cytotoxicity Assays

The measurement and monitoring of cell viability is an essential technique that allows for the optimization of cell culture conditions as well as the determination of cytokine, growth factor, or hormone activity.

• Cell Viability and Cytotoxicity Assay measures both live and dead cells on a fluorescence plate reader; live cells may also be quantified on a standard colorimetric (ELISA) plate reader.
• LDH Cytotoxicity Assay Kit measures and monitors cell cytotoxicity via the release of lactose dehydrogenase from the cell cytoplasm.