NEW! Protein Isolation and Extraction Reagents

We have recently developed a number of reagents and kits to facilitate the extraction and isolation of proteins from cells:

Protein Phosphorylation Reagents

Phosphoproteins represent an important part of cell signaling research. As such, standard methods used in protein analysis are often not sufficient for use with phosphoproteins. We have developed reagents that are specifically designed to improve your phosphoprotein research:

  • PhosphoBLOCKER Blocking Reagent was designed to provide superior blocking compred to dry milk or serum; it enhances low level phosphoprotein signals without increasing background, and it preserves phosphoproteins during Western blotting
  • Our Phosphoprotein Purification Kit provides enrichment of phosphoproteins by affinity purifiction using a single-step purification matrix
  • Our Phospho Antibody Stripping Solution removes phosphoantibodies from blots without affecting the immobilized proteins, allowing you to reprobe with the same or a different antibody  

His-Tag Protein ELISA Kit

Our His-Tag Protein ELISA Kit allows you to detect and quantify His-tagged protein samples simply and reliably by comparing your unknown samples to a known recombinant standard.

Sensitivity range of the kit is 4 µg/mL to 1 ng/mL, or 950 nM to 50 pM of 6xHis-tag residues.

More information  

Antibody Production Tools

If you are producing your own antibodies, you may find the following kits useful:

 

  • Our Rapid Antibody Purification Kit uses a Protein A column to quickly purify your serum, ascites, tissue culture media, or hybridoma supernatants
  • Our Antibody Isotyping Kits give you a quick and easy read on the isotype of your mouse monoclonal antibody; kits read IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, and optional heavy and light chains