Legend: Untreated, Taxol 100nM, or serum restricted (0% FBS) treated A431, or Hela cells were lysed with BlastR lysis buffer (Cytoskeleton Inc.) supplemented with SUMO isopeptidase (deSUMOylase) inhibitor (NEM). 1mg of each lysate was incubated with the recommended amount of SUMO 2/3 affinity beads (ASM24-beads) or IgG control beads (CIG01) (Cytoskeleton Inc.). Immunoprecipitated samples were separated by SDS-PAGE and transferred to PVDF. Western blot was performed with RhoGDI.
Maximize your ability to detect SUMO 2/3 modifications of your target protein
Use the right tools for your SUMO 2/3 investigation:
Affinity reagents: Robust enrichment, capable of endognous SUMO 2/3 detection
Lysis buffers: Inactivates de-SUMOylases (SUMO isopeptidases) through denaturation
Inhibitors: Target and inactivate de-SUMOylases
Western antibody: High sensitivity, detect low % PTMs
Legend: Untreated A431 cells were lysed with BlastR lysis buffer (Cytoskeleton Inc.) supplemented with or without deSUMOylase inhibitor (NEM). 1mg of each lysate was incubated with the recommended amount of SUMO 2/3 affinity beads (ASM24-beads) (Cytoskeleton Inc.). Immunoprecipitated samples were separated by SDS-PAGE and transferred to PVDF. Western blot was performed with SUMO 2/3 antibody (ASM23-HRP), TFII-I, or Ubc9.
It is with great pride and immense pleasure that we introduce the Creative Bioarray QualiStem® Alkaline Phosphatase Live Staining Kit which provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell to be used for follow-up studies
Since the Monkeypox was first detected on 7 May 2022 in the United Kingdom, more and more cases have been confirmed in non-endemic countries, Creative Biogene decided to do our share for supporting the scientific community against this disease and would like to introduce our Monkeypox Virus Real Time PCR Kit.
