Easy to use Oligonucleotide Conjugation Kits

The detection of rare cells or low concentrations of proteins with accuracy and sensitivity in test samples such as whole blood and serum is a significant problem. This is especially true with standard immunoassays such as ELISA.

With the amplification offered by PCR, the detection of single nucleic acid molecules is now routine-a DNA probe hybridized to its template offers high sensitivity and specificity. The next generation of immunoassay reagents are being engineered through linking oligonucleotides to antibodies. Immuno-PCR combines the specificity of antibodies with the amplification power of PCR allowing a 10 to 1000 fold increase in sensitivity. Antibody-oligonucleotide conjugates also have the potential to be the platform tool in multiplexed protein diagnostic assays.


Using standard chemistry and methodology, the linking of an oligonucleotide to an antibody is difficult, time consuming and requires specialist knowledge. To overcome these issues the Thunder-Link® oligo conjugation system has been designed to allow the non-specialist to generate antibody oligonucelotide conjugates easily and efficiently. Furthermore:

  • The inclusion of positive controls enables the end user to confirm that the conjugation chemistry is working correctly.
  • Technology is unidirectional and thus only allows formation of antibody-oligonucleotide complexes and never antibody-antibody or oligonucleotide-oligonucleotide.

Please contact us for more details or go to the manufacturer's website here