Accurate and sensitive user-friendly formats with over 500 citations

Applications for pull-downs

Measure K-Ras, N-Ras, H-Ras, RhoC, Rac3 isoforms

• Active levels of multiple Ras- and Rho-subfamily GTPase isoforms can be detected

Complement G-LISA findings

• Use two different, validated techniques to quantitate active GTPase level.

Applications for G-LISAs

Measure active GTPases in 3-D cell cultures

• See p.15 of the G-LISA technical guide to read about measuring active G-proteins in these cultures.

Measure active GTPases in primary cell lysates

• Measure active GTPases in as little as 5 µg of total cell extract.

Increase accuracy of active GTPase quantitations

• Quantitative numerical output in OD490nm units.

Measure active GTPases in tissue lysates

• Use as little as 1 mm3 of tissue for extraction.

Cited in hundreds of peer- reviewed articles

3-D Cell Culture and G-LISAs: 

Ponik S.M. et al. 2013. RhoA is down-regulated at cell-cell contacts via p190RhoGAP-B in response to tensional homeostasis. Mol. Biol. Cell. 24, 1688-1699. 

Petroll W.M. et al. 2008. Dynamic assessment of fibroblast mechanical activity during Rac-induced cell spreading in 3-D culture. J. Cell Physiol. 217, 162-171. 

Keely P.J. et al. 2007. Investigating integrin regulation and signaling events in three-dimensional systems. Methods Enzymol. 426, 27-45. 

Primary Cells and G-LISAs: 

Valdez C.M. et al. 2016. The Rac-GAP alpha2-chimaerin regulates hippocampal dendrite and spine morphogenesis.  Mol. Cell. Neurosci. 75, 14-26. 

Nini L. and Dagino L. 2010. Accurate and reproducible measurements of RhoA activation in small samples of primary cells. Anal. Biochem. 398, 135-137. 

Flaiz C. et al. 2009. PAK kinase regulates Rac GTPase and is a potential target in human schwannomas. Exp. Neurol. 218, 137-144. 

Increased Accuracy and G-LISAs:

Oliver A.W. et al. 2011. The HPV16 E6 binding protein Tip-1 interacts with ARHGEF16, which activates Cdc42. Br. J. Cancer. 104, 324 - 331. 

GTPase Isoforms and Pull-downs:

Tabibian J.H. et al. 2014. Cholangiocyte senescence by way of N-Ras activation is a characteristic of primary sclerosing cholangitis. Hepatology. 59, 2263-2275. 

Kusuhara S. et al. 2012. Arhgef15 promotes retinal angiogenesis by mediating VEGF-induced Cdc42 activation and potentiating RhoJ inactivation in endothe­lial cells. PLoS ONE. 9:e45858. 

Pull-downs Complementing G-LISAs:

Tanaka T. et al. 2010. Monocyte chemoattractant protein-1/CC chemokine ligand 2 enhances apoptotic cell removal by macrophages through Rac1 activation. Biochem. Biophys. Res. Commun. 399, 677-682. 

Schlegel N. and Waschke J. 2009. VASP is involved in cAMP-mediated Rac 1 activation in microvascular endothelial cells. Am. J. Physiol. Cell Physiol. 296, C453-C462. 

Lai F.P.L. et al. 2009. Cortactin Promotes Migration and Platelet-derived Growth Factor-induced Actin Reorganization by Signaling to Rho-GTPases. Mol. Biol. Cell. 20, 3209-3223.