It is with great pride and immense pleasure that we introduce the Creative Bioarray QualiStem® Alkaline Phosphatase Live Staining Kit which provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell to be used for follow-up studies
Details
Tetramethyl benzidine (TMB) is a noncarcinogenic analog of benzidine, which is used as a substrate for enzyme immunoassays using horseradish peroxidase conjugated antibodies and molecular probes. This novel formulation of TMB employs a non-hazardous, non-toxic solvent system that permits the use of buffers that are more appropriate to the function of the TMB dye reaction. This system develops a blue reaction product when it is reacted with peroxidase conjugates. This can then be read visually at 370 nm and 620 to 650 nm.When the reaction is stopped, the color turns from blue to yellow and is read at 450 nm.
Additional Information
| Suppliers | Exalpha |
|---|---|
| Presentation | 100 ml |