It is with great pride and immense pleasure that we introduce the Creative Bioarray QualiStem® Alkaline Phosphatase Live Staining Kit which provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell to be used for follow-up studies
Details
38 kDa SNAPs (soluble NSF attachment proteins), acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide- sensitive fusion protein (NSF) are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-toendosome fusion and transcytotic vesicles-to-plasma membrane transport. Vesicle-to-target membrane docking (initial contact) occurs when the vesicle SNARE binds to its cognate target membrane SNARE. ?-SNAP (or ?-SNAP in brain) then binds to this docking complex and mediates the binding of NSF and thus the formation of a 20S fusion particle. It is thought that, once NSF is bound, ATP hydrolysis by NSF initiates the fusion process.
Additional Information
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