Tetramethylrhodamine isothiocyanate isomer R

Together with FITC, TRITC is still widely used in fluorescence microscopy. Stable covalent bonds are obtained without destroying the fluorescents the fluorescent structure Unreacted material can easily be removed by dialysis or gel filtration. Fluorescence efficiency is only slightly effected on conjugation to proteins. TRITC- antibody conjugates are stable under normal storage conditions, while immunological properties of antigens and antibodies remain reserved. TRITC, especially the racemic form, may have cytotoxic properties. TRITC-R has been tested for the absence of toxicity. Although its fluorescence efficiency is less than that of FITC, TRITC has the additional advantage of significantly less photo decomposition during excitation which makes it more suitable for cytochemical techniques requiring prolonged microscopic observation. If non-specific staining in histochemistry or cytochemistry is troublesome, a lower F/P ratio may be advisable. This is achieved by reducing the amount of the fluorochrome.