It is with great pride and immense pleasure that we introduce the Creative Bioarray QualiStem® Alkaline Phosphatase Live Staining Kit which provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell to be used for follow-up studies
Details
Recombinant HEK293 cell line expressing human PDE10A2 (phosphodiesterase 10A, accession number NM_006661).
N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels.
N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels.
Additional Information
| Suppliers | BPS Bioscience |
|---|---|
| Presentation | 2 vials |