G Protein-Coupled Receptor 182

G Protein-Coupled Receptor 182has been suggested to be an Adrenomedullin Receptor. Originally cloned from rats and later from humans, GPCR 182 does not to bind adrenomedullin or display functional cAMP response to adrenomedullin (reviewed in Poyner et al. 2002). The initial experimental results, which demonstrated high-affinity binding and increased levels of cAMP in COS cells that were transfected with L1/G10D and treated with adrenomedullin, could not be reproduced by other laboratories (Kapas et al. 1995). It is now known that the functional adrenomedullin receptor is a heterodimeric complex composed of calcitonin receptor-like receptor (CRLR) and receptor activity modifying proteins (RAMPs) (McLatchie et al. 1998). GPCR 182 therefore should be considered an Orphan A receptor.