Cycloscope B-All

Cycloscope B-ALL, is a kit used for the flow cytometric analysis of DNA cell contents in B-lineage acute lymphoblastic leukemia (B-ALL). This kit is mainly focused for DNA studies of leukemic blast cells from bone marrow samples of these patients. Introduction:Acute lymphoblastic leukemia is a disorder characterized by a clonal expansion of lymphoid progenitor cells arrested at different differentation steps whose progressive accumulation causes bone marrow involvement with more than 30% blast cells at diagnosis. According to the European Group for the Immunological Clasification of Leukemias, assignement of an ALL to the B-lineage is based on the expression on leukemic cells of at least two B lineage associated antigens: CD19, cytoplasmic CD79a (mb-1) or cytoplasmic CD22. DNA cell content studies by flow cytometry provide relevant information for the prognostic evaluation and follow-up of patients with acute lymphoblastic leukemia. Detection of hyperdiploid leukemic blast cells is an independent prognostic factor strongly associated with favourable clinical and biologic features (1-3); in addition it may be of great utility for the detection of minimal residual disease contributing to relapse prediction in these patients (4, 5). At present the clinical utility of cell cycle studies in B-lineage ALL still remains to be established (6).