BrdU (Bromodeoxyuridine), FL549

Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.
A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators (1-8). In these methods bromodeoxyuridine (BrdU), a thymidine analog replaces [3H] thymidine. BrdU is incorporated, into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA.
Exalpha Biologicals BrdU Immunofluorescence Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and fluorescent staining of these cells which is achieved using a biotinylated anti-BrdU antibody followed by Streptavidin-FL Conjugate