Phosphotyrosine agarose conjugate

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine-phosphorylated protein biochemical pathways in which they function. The agarose conjugate of clone PY20 anti-phosphotyrosine is especially useful for the isolation of phosphotyrosyl proteins in both immunoprecipitation and affinity chromatography protocols in situations wherein the use of the soluble antibody would mask the protein of interest during subsequent steps.