It is with great pride and immense pleasure that we introduce the Creative Bioarray QualiStem® Alkaline Phosphatase Live Staining Kit which provides a fast, simple and sensitive method for AP detection without fixing the cells. The reagent is nontoxic to the cells and the fluorescent signal will be discharged from the cells by exocytosis completely within two hours after removal of the staining solution, allowing the cell to be used for follow-up studies
Details
MEG-2 also known as PTPN9 is a protein-tyrosine phosphatase originally isolated from peripheral neutrophilic polymorphonuclear leukocytes (PMN). MEG2 is predominantly cytosolic with components present in secondary and tertiary granules and secretory vesicles. MEG2 activity is inhibited after exposure of cells to opsonized zymosan or to phorbol 12-myristate 13-acetate but largely unaffected by the f-met-leu-phe (N-formyl-methionyl-leucyl-phenyalanine). Cysteine 515 is essential for catalytic activity of MEG-2, whereas the noncatalytic (N-terminal) domain of MEG2 negatively regulates the enzymatic activity of the C-terminal phosphatase domain. The activity of MEG2 is enhanced by specific polyphosphoinositides. MEG2 associates at an early stage with nascent phagosomes. MEG2 is a polyphosphoinositide-activated tyrosine phosphatase that may be involved in signaling events regulating phagocytosis, and essential antimicrobial function in the immune response.
Additional Information
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