cytologo

Physiologic Detection: Cytoskeleton's comphrehensive Ubiquitin Detection Kit (Cat. # BK161) simplifies endogenous mono- and poly- ubiquitination detection.

ubiquitinationdetection

Legend: Schematic showing Ubiquitin Detection Kit Assay Flow

Click here to see a recent Nature article utilising the Ubiquitin Detection Kit

 

Maximize your ability to detect mono- and poly- ubiquitinated target proteins

Affinity reagents: Robust enrichment, capable of mono- and poly- Ub detection

Control affinity beads: proper controls for determining positive signals
Lysis buffers: Inactivates isopeptidases through denaturation
Inhibitors: Target and inactivate de-ubiquitinases
Western antibody: High sensitivity, detect low % PTMs
Detection Reagents: Exceptional signal, detect low % PTMs

ubiquitination detection

 

Legend: (A) 3T3 cells pretreated with MG-132 and +/- cytosolic necrotizing factor 1 (CN04) were lysed with BlastR lysis buffer. 300ug of lysate was incubated with the specified amount of the various ubiquitin affinity beads. Immunoprecipitated samples were separated by SDS-PAGE and transferred to PVDF. Western blot was performed with a Rac1 antibody. The lysis system (Cat. # BLR01), Ub affinity beads (Cat. # UBA01-beads), Ub control beads (Cat. # CUB02-beads), and inhibitors are all components of the Ub detection kit (Cat. # BK161).

For more information about our ubiquitin detection tools, see our ubiquitination white paper or click on the links below
 
 

 

Signal Seeker Ubiquitin Detection Tools

 

(BK161) Signal-Seeker Ubiquitination Detection Kits (30 Assays)  

 

(BK 161-S)  Signal-Seeker Ubiquitination Detection Kits (10 Assays)

 

(UBA01-beads) Ubiquitination Affinity Beads

 

(CUB02-beads) Ubiquitination Control Beads

 

(AUB01) Ubiquitin Mouse Monoclonal Antibody 

 

(AUB01-HRP) Ubiquitin-HRP labeled Mouse Monoclonal Antibody

 

(BLR01) BlastR Rapid Lysate Prep Kit