pOET3 combines the flexibility and convenience offered by the pOET1 and pOET2 vectors, with enhanced capabilities that complement the improvements in protein expression delivered by the Company’s successful flashBAC vectors. pOET1 and pOET2 are baculovirus transfer plasmids designed for high-level expression of foreign genes under the powerful AcMNPV polyhedrin (polh) promoter.

pOET3 protein expression is driven from the AcMNPV p6.9 promoter, enabling earlier expression compared with the polh promoter used in pOET1 and pOET2. The recombinant protein is therefore produced at a time during the infection process when the cellular machinery is in a much healthier condition, compared to late times in infection.  This can give significant improvements when proteins that require extensive post-translational modifications are produced, such as glycoproteins destined for secretion or membrane insertion.

pOET3 plasmids are also:

• Compatible with all baculovirus systems based on homologous recombination.
• Have the same MCS and small size as pOET1 to facilitate easy cloning.

The release of pOET3 follows Oxford Expression Technologies’ launch of its successful suite of transfer plasmids with unique signal sequences and promoters, including pOET1 and pOET1N that increase cloning efficiency and expression of foreign genes.