Newsletter April 2010

Try an antibody for free - with compliments of Everest Biotech Everest Biotech would like to invite all scientists to test these antibodies in appropriate applications. Therefore we offer all our Aspiring products free of charge. To find out more about Aspiring grade product please click here, to see an example please view this link. Terms and condition apply - Participation must be approved by Everest Biotech (please contact support@everestbiotech.com) - Data must be returned within 3 months of receipt of antibody. Everest Biotech Ltd reserves the right to charge full price of the Aspiring Antibody if no data is returned within 3 months - All images and data may be used by Everest Biotech for marketing purposes (with acknowledgment, unless you wish to remain anonymous)  - Shipping cost to be carried by laboratory - One free vial per laboratory New Products This month we have released the following antibodies: Afamin / alpha Albumin, CDO, CYB5R3 / Dia 1 (mouse), elF5A, FAS / CD95, GGCX, GLUT5 / SLC2A5, IP6K3, IREB2 / IRP2, Klk6/ neurosin (mouse), NEBL / nebulette, NUDC, PREX1, RAP80, SCNN1D, SDHAF1, SS-56 / TRIM68, Talin 1 / TLN1, TFB1M, TMPRSS11D / HAT, TPCN2, URP2 / kindlin-3, WNT15 / WNT9B, Featured Products EB05859 Goat Anti-NEIL1 / NEH1 Antibody NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair after DNA damage. Quantitative PCR of mouse tissues detected Neil1 expression in all tissues examined, with highest expression in spleen, heart, and lung. Secondary Antibody Rabbit Anti-Goat IgG Antibody HRP conjugated For more information please contact our technical support team.  Matching Immunizing Peptides available For more information please contact our technical support team.

Focus area: DNA Repair DNA is continuously prone to damage either by metabolism, or by environmental factors. Both nuclear and mitochondrial DNA can be affected. To maintain cellular integrity, the DNA repair machinery is constantly active as it responds to damage in the DNA structure. When this mechanism fails the cell could become senescent, it could enter apoptosis, or it could become cancerous. The PARP family members are essential in a number of cellular functions. One important function of PARP is assisting in the repair of single-strand DNA nicks. PARP binds sites with single strand breaks through its N-terminal zinc fingers and will recruit XRCC1, DNA ligase III, DNA polymerase beta and a kinase to the nick. This process is called base excision repair (BER). BRCA repairs double strand breaks, but when BRCA is impaired, PARP can take over. PARP inhibitors are currently being investigated as agents for chemotherapy, particularly for breast cancer. Everest Biotech continues to expand its catalogue with antibodies to proteins involving DNA repair. Please visit our website to see all our current products on DNA repair. New IHC Data EB06206 Goat Anti-POLL / BETA-N Antibody Product Focus EB05865 Goat Anti-ALS2CR2 / ILPIP / STRADB Antibody ALS2CR2 / ILPIP / STRADB belongs to the serine/threonine protein kinase STE20 subfamily. This protein is a pseudokinase because one of the active site residues in the protein kinase domain is altered. In complex with CAB39 / MO25, this pseudokinase binds to and activates serine/threonine kinase 11 (STK11), a master kinase that regulates cell polarity and energy-generating metabolism. It relocates STK11 from the nucleus to the cytoplasm and plays an essential role in STK-11 mediated G1 cell cycle arrest. The protein can also interact with the X chromosome-linked inhibitor of apoptosis protein, therefore enhancing the anti-apoptotic activity of this protein via the JNK1 signal transduction pathway. This protein is highly expressed in heart, skeletal muscle, testis, liver and colon